Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors
Abstract Objective Pleural effusion (PE) is a common feature of malignant pleural mesothelioma. These effusions typically contain lymphocytes and malignant cells. We postulated that the PE would be a source of lymphocytes for analysis of tumor immune milieu. The aim of this study was to compare the...
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doaj-d41151ec53c34c3380c590cd7d030f322020-11-25T01:18:33ZengBMCBMC Research Notes1756-05002018-12-011111610.1186/s13104-018-3953-xTumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptorsJonathan Chee0Mark W. Watson1Abha Chopra2Bella Nguyen3Alistair M. Cook4Jenette Creaney5Willem J. Lesterhuis6Bruce W. Robinson7Y. C. Gary Lee8Anna K. Nowak9Richard A. Lake10Alison M. McDonnell11National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaInstitute for Immunology and Infectious Diseases, Murdoch UniversityInstitute for Immunology and Infectious Diseases, Murdoch UniversityDepartment of Medical Oncology, Sir Charles Gairdner HospitalNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaDepartment of Respiratory Medicine, Sir Charles Gairdner HospitalNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaNational Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western AustraliaAbstract Objective Pleural effusion (PE) is a common feature of malignant pleural mesothelioma. These effusions typically contain lymphocytes and malignant cells. We postulated that the PE would be a source of lymphocytes for analysis of tumor immune milieu. The aim of this study was to compare the phenotype and T cell receptor usage of pleural effusion T cells with paired concurrently drawn peripheral blood lymphocytes. We used multi-parameter flow cytometry and high-throughput T cell receptor sequencing to analyse peripheral blood and pleural effusion mononuclear cells. Results Both CD8+ and CD4+ T cells from effusion showed increased expression of T cell inhibitory receptors PD-1, LAG-3 and Tim-3 compared to blood. Comprehensive T cell receptor sequencing on one of the patients showed a discordant distribution of clonotypes in the antigen-experienced (PD-1+) compartment between effusion and blood, suggesting an enrichment of antigen specific clonotypes in the effusion, with potential as an immunological response biomarker.http://link.springer.com/article/10.1186/s13104-018-3953-xMesotheliomaPleural effusionT cell receptor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonathan Chee Mark W. Watson Abha Chopra Bella Nguyen Alistair M. Cook Jenette Creaney Willem J. Lesterhuis Bruce W. Robinson Y. C. Gary Lee Anna K. Nowak Richard A. Lake Alison M. McDonnell |
spellingShingle |
Jonathan Chee Mark W. Watson Abha Chopra Bella Nguyen Alistair M. Cook Jenette Creaney Willem J. Lesterhuis Bruce W. Robinson Y. C. Gary Lee Anna K. Nowak Richard A. Lake Alison M. McDonnell Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors BMC Research Notes Mesothelioma Pleural effusion T cell receptor |
author_facet |
Jonathan Chee Mark W. Watson Abha Chopra Bella Nguyen Alistair M. Cook Jenette Creaney Willem J. Lesterhuis Bruce W. Robinson Y. C. Gary Lee Anna K. Nowak Richard A. Lake Alison M. McDonnell |
author_sort |
Jonathan Chee |
title |
Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
title_short |
Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
title_full |
Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
title_fullStr |
Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
title_full_unstemmed |
Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
title_sort |
tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2018-12-01 |
description |
Abstract Objective Pleural effusion (PE) is a common feature of malignant pleural mesothelioma. These effusions typically contain lymphocytes and malignant cells. We postulated that the PE would be a source of lymphocytes for analysis of tumor immune milieu. The aim of this study was to compare the phenotype and T cell receptor usage of pleural effusion T cells with paired concurrently drawn peripheral blood lymphocytes. We used multi-parameter flow cytometry and high-throughput T cell receptor sequencing to analyse peripheral blood and pleural effusion mononuclear cells. Results Both CD8+ and CD4+ T cells from effusion showed increased expression of T cell inhibitory receptors PD-1, LAG-3 and Tim-3 compared to blood. Comprehensive T cell receptor sequencing on one of the patients showed a discordant distribution of clonotypes in the antigen-experienced (PD-1+) compartment between effusion and blood, suggesting an enrichment of antigen specific clonotypes in the effusion, with potential as an immunological response biomarker. |
topic |
Mesothelioma Pleural effusion T cell receptor |
url |
http://link.springer.com/article/10.1186/s13104-018-3953-x |
work_keys_str_mv |
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