Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis
Numerous studies have documented ultrastructural abnormalities in malignant megakaryocytes from bone marrow (BM) of myelofibrosis patients but the morphology of these cells in spleen, an important extramedullary site in this disease, was not investigated as yet. By transmission-electron microscopy,...
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doaj-d41a049fd3d640b1949fe63a97d433392020-11-25T04:02:18ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-11-011010.3389/fonc.2020.584541584541Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With MyelofibrosisMaria Zingariello0Vittorio Rosti1Alessandro M. Vannucchi2Paola Guglielmelli3Maria Mazzarini4Giovanni Barosi5Maria Luisa Genova6Anna Rita Migliaccio7Anna Rita Migliaccio8Unit of Microscopic and Ultrastructural Anatomy, Department of Medicine, University Campus Bio-Medico, Rome, ItalyCenter for the Study of Myelofibrosis, Laboratory of Biochemistry Biotechnology and Advanced Diagnosis, IRCCS Policlinico San Matteo, Pavia, ItalyCRIMM; Center Research and Innovation of Myeloproliferative Neoplasms, AOUC, University of Florence, Florence, ItalyCRIMM; Center Research and Innovation of Myeloproliferative Neoplasms, AOUC, University of Florence, Florence, ItalyBiomedical and Neuromotor Sciences, Alma Mater University Bologna, Bologna, ItalyCenter for the Study of Myelofibrosis, Laboratory of Biochemistry Biotechnology and Advanced Diagnosis, IRCCS Policlinico San Matteo, Pavia, ItalyBiomedical and Neuromotor Sciences, Alma Mater University Bologna, Bologna, ItalyBiomedical and Neuromotor Sciences, Alma Mater University Bologna, Bologna, ItalyMyeloproliferative Neoplasm-Research Consortium, New York, NY, United StatesNumerous studies have documented ultrastructural abnormalities in malignant megakaryocytes from bone marrow (BM) of myelofibrosis patients but the morphology of these cells in spleen, an important extramedullary site in this disease, was not investigated as yet. By transmission-electron microscopy, we compared the ultrastructural features of megakaryocytes from BM and spleen of myelofibrosis patients and healthy controls. The number of megakaryocytes was markedly increased in both BM and spleen. However, while most of BM megakaryocytes are immature, those from spleen appear mature with well-developed demarcation membrane systems (DMS) and platelet territories and are surrounded by platelets. In BM megakaryocytes, paucity of DMS is associated with plasma (thick with protrusions) and nuclear (dilated with large pores) membrane abnormalities and presence of numerous glycosomes, suggesting a skewed metabolism toward insoluble polyglucosan accumulation. By contrast, the membranes of the megakaryocytes from the spleen were normal but these cells show mitochondria with reduced crests, suggesting deficient aerobic energy-metabolism. These distinctive morphological features suggest that malignant megakaryocytes from BM and spleen express distinctive metabolic impairments that may play different roles in the pathogenesis of myelofibrosis.https://www.frontiersin.org/articles/10.3389/fonc.2020.584541/fullmyeloproliferative neoplasmsmyelofibrosismegakaryocytesultrastructural analysescell metabolism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Zingariello Vittorio Rosti Alessandro M. Vannucchi Paola Guglielmelli Maria Mazzarini Giovanni Barosi Maria Luisa Genova Anna Rita Migliaccio Anna Rita Migliaccio |
spellingShingle |
Maria Zingariello Vittorio Rosti Alessandro M. Vannucchi Paola Guglielmelli Maria Mazzarini Giovanni Barosi Maria Luisa Genova Anna Rita Migliaccio Anna Rita Migliaccio Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis Frontiers in Oncology myeloproliferative neoplasms myelofibrosis megakaryocytes ultrastructural analyses cell metabolism |
author_facet |
Maria Zingariello Vittorio Rosti Alessandro M. Vannucchi Paola Guglielmelli Maria Mazzarini Giovanni Barosi Maria Luisa Genova Anna Rita Migliaccio Anna Rita Migliaccio |
author_sort |
Maria Zingariello |
title |
Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis |
title_short |
Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis |
title_full |
Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis |
title_fullStr |
Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis |
title_full_unstemmed |
Shared and Distinctive Ultrastructural Abnormalities Expressed by Megakaryocytes in Bone Marrow and Spleen From Patients With Myelofibrosis |
title_sort |
shared and distinctive ultrastructural abnormalities expressed by megakaryocytes in bone marrow and spleen from patients with myelofibrosis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-11-01 |
description |
Numerous studies have documented ultrastructural abnormalities in malignant megakaryocytes from bone marrow (BM) of myelofibrosis patients but the morphology of these cells in spleen, an important extramedullary site in this disease, was not investigated as yet. By transmission-electron microscopy, we compared the ultrastructural features of megakaryocytes from BM and spleen of myelofibrosis patients and healthy controls. The number of megakaryocytes was markedly increased in both BM and spleen. However, while most of BM megakaryocytes are immature, those from spleen appear mature with well-developed demarcation membrane systems (DMS) and platelet territories and are surrounded by platelets. In BM megakaryocytes, paucity of DMS is associated with plasma (thick with protrusions) and nuclear (dilated with large pores) membrane abnormalities and presence of numerous glycosomes, suggesting a skewed metabolism toward insoluble polyglucosan accumulation. By contrast, the membranes of the megakaryocytes from the spleen were normal but these cells show mitochondria with reduced crests, suggesting deficient aerobic energy-metabolism. These distinctive morphological features suggest that malignant megakaryocytes from BM and spleen express distinctive metabolic impairments that may play different roles in the pathogenesis of myelofibrosis. |
topic |
myeloproliferative neoplasms myelofibrosis megakaryocytes ultrastructural analyses cell metabolism |
url |
https://www.frontiersin.org/articles/10.3389/fonc.2020.584541/full |
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