Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect
<i>Glycyrrhizae Radix et Rhizoma</i> (GRR) is one of the commonly used traditional Chinese medicines in clinical practice, which has been applied to treat digestive system diseases for hundreds of years. GRR is preferred for anti-gastric ulcer, however, the main active compounds are stil...
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doaj-d422977e5bac4b79bdb1a4a5430c83922021-03-11T00:03:46ZengMDPI AGMolecules1420-30492021-03-01261512151210.3390/molecules26061512Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer EffectYuhan Liu0Meiling Zhang1Jinjun Cheng2Yue Zhang3Hui Kong4Yan Zhao5Huihua Qu6School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, ChinaCenter of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China<i>Glycyrrhizae Radix et Rhizoma</i> (GRR) is one of the commonly used traditional Chinese medicines in clinical practice, which has been applied to treat digestive system diseases for hundreds of years. GRR is preferred for anti-gastric ulcer, however, the main active compounds are still unknown. In this study, GRR was used as precursor to synthesize carbon dots (CDs) by a environment-friendly one-step pyrolysis process. GRR-CDs were characterized by using transmission electron microscopy, high-resolution TEM, fourier transform infrared, ultraviolet-visible and fluorescence spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. In addition, cellular toxicity of GRR-CDs was studied by using CCK-8 in RAW264.7 cells, and the anti-gastric ulcer activity was evaluated and confirmed using mice model of acute alcoholic gastric ulcer. The experiment confirmed that GRR-CDs were the spherical structure with a large number of active groups on the surface and their particle size ranged from 2 to 10 nm. GRR-CDs had no toxicity to RAW264.7 cells at concentration of 19.5 to 5000 μg/mL and could reduce the oxidative damage of gastric mucosa and tissues caused by alcohol, as demonstrated by restoring expression of malondialdehyde, superoxide dismutase and nitric oxide in serum and tissue of mice. The results indicated the explicit anti-ulcer activity of GRR-CDs, which provided a new insights for the research on effective material basis of GRR.https://www.mdpi.com/1420-3049/26/6/1512<i>Glycyrrhizae Radix et Rhizoma</i>carbon dotsacute alcoholic gastric ulcerprotective effect |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuhan Liu Meiling Zhang Jinjun Cheng Yue Zhang Hui Kong Yan Zhao Huihua Qu |
spellingShingle |
Yuhan Liu Meiling Zhang Jinjun Cheng Yue Zhang Hui Kong Yan Zhao Huihua Qu Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect Molecules <i>Glycyrrhizae Radix et Rhizoma</i> carbon dots acute alcoholic gastric ulcer protective effect |
author_facet |
Yuhan Liu Meiling Zhang Jinjun Cheng Yue Zhang Hui Kong Yan Zhao Huihua Qu |
author_sort |
Yuhan Liu |
title |
Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect |
title_short |
Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect |
title_full |
Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect |
title_fullStr |
Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect |
title_full_unstemmed |
Novel Carbon Dots Derived from <i>Glycyrrhizae Radix et Rhizoma</i> and Their Anti-Gastric Ulcer Effect |
title_sort |
novel carbon dots derived from <i>glycyrrhizae radix et rhizoma</i> and their anti-gastric ulcer effect |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2021-03-01 |
description |
<i>Glycyrrhizae Radix et Rhizoma</i> (GRR) is one of the commonly used traditional Chinese medicines in clinical practice, which has been applied to treat digestive system diseases for hundreds of years. GRR is preferred for anti-gastric ulcer, however, the main active compounds are still unknown. In this study, GRR was used as precursor to synthesize carbon dots (CDs) by a environment-friendly one-step pyrolysis process. GRR-CDs were characterized by using transmission electron microscopy, high-resolution TEM, fourier transform infrared, ultraviolet-visible and fluorescence spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction and high-performance liquid chromatography. In addition, cellular toxicity of GRR-CDs was studied by using CCK-8 in RAW264.7 cells, and the anti-gastric ulcer activity was evaluated and confirmed using mice model of acute alcoholic gastric ulcer. The experiment confirmed that GRR-CDs were the spherical structure with a large number of active groups on the surface and their particle size ranged from 2 to 10 nm. GRR-CDs had no toxicity to RAW264.7 cells at concentration of 19.5 to 5000 μg/mL and could reduce the oxidative damage of gastric mucosa and tissues caused by alcohol, as demonstrated by restoring expression of malondialdehyde, superoxide dismutase and nitric oxide in serum and tissue of mice. The results indicated the explicit anti-ulcer activity of GRR-CDs, which provided a new insights for the research on effective material basis of GRR. |
topic |
<i>Glycyrrhizae Radix et Rhizoma</i> carbon dots acute alcoholic gastric ulcer protective effect |
url |
https://www.mdpi.com/1420-3049/26/6/1512 |
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