mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis.
The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infect...
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Via Medica
2005-02-01
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Series: | Folia Histochemica et Cytobiologica |
Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4639 |
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doaj-d4265e75e5284418bd371ba3a308bd542020-11-25T01:04:18ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312005-02-0142420921310.5603/4639mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis.Jan ZeromskiIwona Mozer-LisewskaMagdalena FrydrychowiczAndrzej WierzbickiElzbieta Wandurska-NowakKrystyna BoczońThe aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis.http://czasopisma.viamedica.pl/fhc/article/view/4639 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jan Zeromski Iwona Mozer-Lisewska Magdalena Frydrychowicz Andrzej Wierzbicki Elzbieta Wandurska-Nowak Krystyna Boczoń |
spellingShingle |
Jan Zeromski Iwona Mozer-Lisewska Magdalena Frydrychowicz Andrzej Wierzbicki Elzbieta Wandurska-Nowak Krystyna Boczoń mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. Folia Histochemica et Cytobiologica |
author_facet |
Jan Zeromski Iwona Mozer-Lisewska Magdalena Frydrychowicz Andrzej Wierzbicki Elzbieta Wandurska-Nowak Krystyna Boczoń |
author_sort |
Jan Zeromski |
title |
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. |
title_short |
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. |
title_full |
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. |
title_fullStr |
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. |
title_full_unstemmed |
mRNA expression and immunohistochemical localization of inducible nitric oxide synthase (NOS-2) in the muscular niche of Trichinella spiralis. |
title_sort |
mrna expression and immunohistochemical localization of inducible nitric oxide synthase (nos-2) in the muscular niche of trichinella spiralis. |
publisher |
Via Medica |
series |
Folia Histochemica et Cytobiologica |
issn |
0239-8508 1897-5631 |
publishDate |
2005-02-01 |
description |
The aim of this study was to demonstrate iNOS mRNA expression in muscular phase of experimental trichinellosis and to localize iNOS protein in T. spiralis-infected muscles using specific anti-iNOS monoclonal antibodies. The expression of iNOS mRNA in skeletal muscles from Trichinella spiralis-infected mice was examined using the reverse transcription PCR assay. Fragments of skeletal muscles were also subjected to the immunohistochemical reaction using specific anti-iNOS monoclonal antibodies followed by Dako-Ark test. mRNA for iNOS measured on day 21 after infection was expressed in the muscular phase of trichinellosis. Positive immunostaining for iNOS occurred in infiltrating mononuclear cells around the encapsulated larvae. iNOS-positive cells could be traced from the 21st day post infection (dpi); on 42 dpi and 90 dpi most cells expressed iNOS. By assessing expression of protein and its mRNA it can be concluded that iNOS is active in the pathology of skeletal muscle tissue in experimental trichinellosis. |
url |
http://czasopisma.viamedica.pl/fhc/article/view/4639 |
work_keys_str_mv |
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