Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review
Extracellular polymeric substances (EPS) are one of the main components of biofilm, prompting biofilm to form a cohesive three-dimensional framework. Numerous methods are available to help characterize the properties and the structural, chemical and physical organizations of EPS during the biofilm f...
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North Carolina State University
2016-08-01
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doaj-d42cef1dec41402b843f039c7702f7802020-11-25T00:26:42ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-08-011138092811510.15376/biores.11.3.8092-8115Detection Techniques for Extracellular Polymeric Substances in Biofilms: A ReviewMei Pan0Liang Zhu1Lin Chen2Yunpeng Qiu3Jun Wang4State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China; College of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224003, China; ChinaState Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China; ChinaState Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China; ChinaState Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China; ChinaState Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China; ChinaExtracellular polymeric substances (EPS) are one of the main components of biofilm, prompting biofilm to form a cohesive three-dimensional framework. Numerous methods are available to help characterize the properties and the structural, chemical and physical organizations of EPS during the biofilm formation process. This review highlights key techniques from different disciplines that have been successfully applied in-situ and non-destructively to describe the complex composition and distribution of EPS in biofilm, especially microscopic, spectroscopic, and the combination of multi-disciplinary methods that can provide new insights into the complex structure/function correlations in biofilms. Among them, confocal laser scanning microscopy (CLSM) is emphasized, and its principles, applications, advantages, and limitations are summarized. Multidisciplinary techniques have been developed and recommended to study EPS during the biofilm formation process, providing more in-depth insights into the composition and spatial distributions of EPS, so as to improve our understanding of the role EPS plays in biofilms ultimately.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_3_Pan_Review_8092_Detection_Techniques_Extracellular_BiofilmsExtracellular polymeric substanceBiofilmDetection techniqueMultidisciplinary methods |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mei Pan Liang Zhu Lin Chen Yunpeng Qiu Jun Wang |
spellingShingle |
Mei Pan Liang Zhu Lin Chen Yunpeng Qiu Jun Wang Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review BioResources Extracellular polymeric substance Biofilm Detection technique Multidisciplinary methods |
author_facet |
Mei Pan Liang Zhu Lin Chen Yunpeng Qiu Jun Wang |
author_sort |
Mei Pan |
title |
Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review |
title_short |
Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review |
title_full |
Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review |
title_fullStr |
Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review |
title_full_unstemmed |
Detection Techniques for Extracellular Polymeric Substances in Biofilms: A Review |
title_sort |
detection techniques for extracellular polymeric substances in biofilms: a review |
publisher |
North Carolina State University |
series |
BioResources |
issn |
1930-2126 1930-2126 |
publishDate |
2016-08-01 |
description |
Extracellular polymeric substances (EPS) are one of the main components of biofilm, prompting biofilm to form a cohesive three-dimensional framework. Numerous methods are available to help characterize the properties and the structural, chemical and physical organizations of EPS during the biofilm formation process. This review highlights key techniques from different disciplines that have been successfully applied in-situ and non-destructively to describe the complex composition and distribution of EPS in biofilm, especially microscopic, spectroscopic, and the combination of multi-disciplinary methods that can provide new insights into the complex structure/function correlations in biofilms. Among them, confocal laser scanning microscopy (CLSM) is emphasized, and its principles, applications, advantages, and limitations are summarized. Multidisciplinary techniques have been developed and recommended to study EPS during the biofilm formation process, providing more in-depth insights into the composition and spatial distributions of EPS, so as to improve our understanding of the role EPS plays in biofilms ultimately. |
topic |
Extracellular polymeric substance Biofilm Detection technique Multidisciplinary methods |
url |
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_3_Pan_Review_8092_Detection_Techniques_Extracellular_Biofilms |
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