Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum
<b>Objective:</b>Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the di...
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doaj-d43ddf7e29834b82acea08bf71e5ad5b2020-11-25T00:16:20ZengChina Anti-Cancer AssociationCancer Biology & Medicine2095-39412095-39412015-09-0112324625410.7497/j.issn.2095-3941.2015.00102015000010Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serumHua-Jun Gao0Ya-Jing Chen1Duo Zuo2Ming-Ming Xiao3Ying Li4Hua Guo5Ning Zhang6Rui-Bing Chen7Research Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;Research Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;Laboratory of Cancer Cell Biology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, ChinaResearch Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;Research Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;Laboratory of Cancer Cell Biology, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, ChinaResearch Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;Research Center of Basic Medical Sciences & School of Medical Laboratory, Tianjin Medical University, Tianjin 300070, China;<b>Objective:</b>Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples.<br/><b>Methods:</b>Lectin affinity chromatography (LAC) was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography (LC) separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins.<br/><b>Results:</b>A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain (FGG), FOS-like antigen 2 (FOSL2), and α-1, 6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase B (MGAT5B) were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples.<br/><b>Conclusion:</b>A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC.http://www.cancerbiomed.org/index.php/cocr/article/view/878Glycoproteinhepatocellular carcinoma (HCC)mass spectrometryproteomicsserum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hua-Jun Gao Ya-Jing Chen Duo Zuo Ming-Ming Xiao Ying Li Hua Guo Ning Zhang Rui-Bing Chen |
spellingShingle |
Hua-Jun Gao Ya-Jing Chen Duo Zuo Ming-Ming Xiao Ying Li Hua Guo Ning Zhang Rui-Bing Chen Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum Cancer Biology & Medicine Glycoprotein hepatocellular carcinoma (HCC) mass spectrometry proteomics serum |
author_facet |
Hua-Jun Gao Ya-Jing Chen Duo Zuo Ming-Ming Xiao Ying Li Hua Guo Ning Zhang Rui-Bing Chen |
author_sort |
Hua-Jun Gao |
title |
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
title_short |
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
title_full |
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
title_fullStr |
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
title_full_unstemmed |
Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
title_sort |
quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum |
publisher |
China Anti-Cancer Association |
series |
Cancer Biology & Medicine |
issn |
2095-3941 2095-3941 |
publishDate |
2015-09-01 |
description |
<b>Objective:</b>Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity of serum screening for early HCC diagnosis. This study employed a quantitative proteomic strategy to analyze the differential expression of serum glycoproteins between HCC and normal control serum samples.<br/><b>Methods:</b>Lectin affinity chromatography (LAC) was used to enrich glycoproteins from the serum samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling and 2D liquid chromatography (LC) separations were performed to examine the differential levels of the detected proteins between HCC and control serum samples. Western blot was used to analyze the differential expression levels of the three serum proteins.<br/><b>Results:</b>A total of 2,280 protein groups were identified in the serum samples from HCC patients by using the 2D LC-MS/MS method. Up to 36 proteins were up-regulated in the HCC serum, whereas 19 proteins were down-regulated. Three differential glycoproteins, namely, fibrinogen gamma chain (FGG), FOS-like antigen 2 (FOSL2), and α-1, 6-mannosylglycoprotein 6-β-N-acetylglucosaminyltransferase B (MGAT5B) were validated by Western blot. All these three proteins were up-regulated in the HCC serum samples.<br/><b>Conclusion:</b>A quantitative glycoproteomic method was established and proven useful to determine potential novel biomarkers for HCC. |
topic |
Glycoprotein hepatocellular carcinoma (HCC) mass spectrometry proteomics serum |
url |
http://www.cancerbiomed.org/index.php/cocr/article/view/878 |
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