Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum
The dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two F. oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible...
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doaj-d440b0e291254ab0aa5b25f8ac81e8d92020-11-24T22:35:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-04-01610.3389/fmicb.2015.00234133659Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporumJonathan eNiño-Sánchez0Vega eTello1Virginia eCasado-del Castillo2Michael R. Thon3Ernesto P Benito4José María Díaz-Mínguez5University of Salamanca CIALEUniversity of Salamanca CIALEUniversity of Salamanca CIALEUniversity of Salamanca CIALEUniversity of Salamanca CIALEUniversity of Salamanca CIALEThe dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two F. oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible common bean cultivar. The growth of the two strains on the root surface and the colonization of the root was cuantitatively similar although the highly virulent strain was more efficient reaching the central root cylinder. The main differences between both strains were found in the temporal and spatial dynamics of crown root and hypocotyl colonization. The increase of fungal biomass in the crown root was considerably larger for the highly virulent strain, which, after an initial stage of global colonization of both the vascular cylinder and the parenchymal cells, restricted its growth to the newly differentiated xylem vessels. The weakly virulent strain was a much slower and less efficient colonizer of the xylem vessels, showing also growth in the intercellular spaces of the parenchyma. Most of the virulence genes analyzed showed similar expression patterns in both strains, except SIX1, SIX6 and the gene encoding the transcription factor FTF1, which were highly upregulated in root crown and hypocotyl. The response induced in the infected plant showed interesting differences for both strains. The weakly virulent strain induced an early and strong transcription of the PR1 gene, involved in SAR response, while the highly virulent strain preferentially induced the early expression of the ethylene responsive factor ERF2.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00234/fullVirulenceconfocal microscopyeffectorPhaseolus vulgarisFusarium oxysporum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jonathan eNiño-Sánchez Vega eTello Virginia eCasado-del Castillo Michael R. Thon Ernesto P Benito José María Díaz-Mínguez |
spellingShingle |
Jonathan eNiño-Sánchez Vega eTello Virginia eCasado-del Castillo Michael R. Thon Ernesto P Benito José María Díaz-Mínguez Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum Frontiers in Microbiology Virulence confocal microscopy effector Phaseolus vulgaris Fusarium oxysporum |
author_facet |
Jonathan eNiño-Sánchez Vega eTello Virginia eCasado-del Castillo Michael R. Thon Ernesto P Benito José María Díaz-Mínguez |
author_sort |
Jonathan eNiño-Sánchez |
title |
Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum |
title_short |
Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum |
title_full |
Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum |
title_fullStr |
Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum |
title_full_unstemmed |
Gene expression patterns and dynamics of the colonization of common bean (Phaseolus vulgaris L.) by highly virulent and weakly virulent strains of Fusarium oxysporum |
title_sort |
gene expression patterns and dynamics of the colonization of common bean (phaseolus vulgaris l.) by highly virulent and weakly virulent strains of fusarium oxysporum |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2015-04-01 |
description |
The dynamics of root and hypocotyl colonization, and the gene expression patterns of several fungal virulence factors and plant defense factors have been analyzed and compared in the interaction of two F. oxysporum f. sp. phaseoli strains displaying clear differences in virulence, with a susceptible common bean cultivar. The growth of the two strains on the root surface and the colonization of the root was cuantitatively similar although the highly virulent strain was more efficient reaching the central root cylinder. The main differences between both strains were found in the temporal and spatial dynamics of crown root and hypocotyl colonization. The increase of fungal biomass in the crown root was considerably larger for the highly virulent strain, which, after an initial stage of global colonization of both the vascular cylinder and the parenchymal cells, restricted its growth to the newly differentiated xylem vessels. The weakly virulent strain was a much slower and less efficient colonizer of the xylem vessels, showing also growth in the intercellular spaces of the parenchyma. Most of the virulence genes analyzed showed similar expression patterns in both strains, except SIX1, SIX6 and the gene encoding the transcription factor FTF1, which were highly upregulated in root crown and hypocotyl. The response induced in the infected plant showed interesting differences for both strains. The weakly virulent strain induced an early and strong transcription of the PR1 gene, involved in SAR response, while the highly virulent strain preferentially induced the early expression of the ethylene responsive factor ERF2. |
topic |
Virulence confocal microscopy effector Phaseolus vulgaris Fusarium oxysporum |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00234/full |
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