Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb
<i>Lobelia chinensis</i> is a kind of herbal medicine widely distributed and used in Asia. The chemical components of this herb, however, have not been well studied until now. Lobeline, as an essential and famous bioactive compound in <i>Lobelia</i> genus, has been assumed to...
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doaj-d449ad3b4567401b9b8cbda39c0a0c152020-11-25T00:14:40ZengMDPI AGMolecules1420-30492018-12-012312325810.3390/molecules23123258molecules23123258Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the HerbHaixing Wang0Yuanyuan Li1Yeqing Huang2Chunyan Zhao3Hon-Yeung Cheung4Department of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China<i>Lobelia chinensis</i> is a kind of herbal medicine widely distributed and used in Asia. The chemical components of this herb, however, have not been well studied until now. Lobeline, as an essential and famous bioactive compound in <i>Lobelia</i> genus, has been assumed to be present in <i>L. chinensis</i>. In order to ascertain its presence and, more importantly, proper use of this herb, chemical profiling this herb with highly sensitive and high-resolution analytical mass spectrometry was applied. In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) method was employed to systematically profile the chemical constituents of <i>L. chinensis</i> for the first time. Comparative chemical profiling study of <i>L. chinensis</i> and <i>Lobelia inflata</i> was also conducted to provide evidence whether lobeline is present or not. Piperidine alkaloids except for lobeline, alkaloid-lignan hybrids, flavonoids, polyacetylenes, nonanedioic acid, and some new phytochemicals were successfully identified in <i>L. chinensis</i> simultaneously. Comparing to the chemical profiles of <i>L. inflata</i>, lobeline was found to be absent in <i>L. chinensis</i>. All of the secondary metabolites in <i>L. chinensis</i> were determined with the HPLC/Q-TOF MS method. The absence of lobeline in <i>L. chinensis</i> was confirmed after this extensive study.https://www.mdpi.com/1420-3049/23/12/3258<i>Lobelia chinensis</i><i>Lobelia inflata</i>lobelineliquid chromatographymass spectrometrychemical profiling |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Haixing Wang Yuanyuan Li Yeqing Huang Chunyan Zhao Hon-Yeung Cheung |
spellingShingle |
Haixing Wang Yuanyuan Li Yeqing Huang Chunyan Zhao Hon-Yeung Cheung Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb Molecules <i>Lobelia chinensis</i> <i>Lobelia inflata</i> lobeline liquid chromatography mass spectrometry chemical profiling |
author_facet |
Haixing Wang Yuanyuan Li Yeqing Huang Chunyan Zhao Hon-Yeung Cheung |
author_sort |
Haixing Wang |
title |
Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb |
title_short |
Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb |
title_full |
Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb |
title_fullStr |
Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb |
title_full_unstemmed |
Chemical Profiling of <i>Lobelia chinensis</i> with High-Performance Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (HPLC/Q-TOF MS) Reveals Absence of Lobeline in the Herb |
title_sort |
chemical profiling of <i>lobelia chinensis</i> with high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (hplc/q-tof ms) reveals absence of lobeline in the herb |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-12-01 |
description |
<i>Lobelia chinensis</i> is a kind of herbal medicine widely distributed and used in Asia. The chemical components of this herb, however, have not been well studied until now. Lobeline, as an essential and famous bioactive compound in <i>Lobelia</i> genus, has been assumed to be present in <i>L. chinensis</i>. In order to ascertain its presence and, more importantly, proper use of this herb, chemical profiling this herb with highly sensitive and high-resolution analytical mass spectrometry was applied. In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) method was employed to systematically profile the chemical constituents of <i>L. chinensis</i> for the first time. Comparative chemical profiling study of <i>L. chinensis</i> and <i>Lobelia inflata</i> was also conducted to provide evidence whether lobeline is present or not. Piperidine alkaloids except for lobeline, alkaloid-lignan hybrids, flavonoids, polyacetylenes, nonanedioic acid, and some new phytochemicals were successfully identified in <i>L. chinensis</i> simultaneously. Comparing to the chemical profiles of <i>L. inflata</i>, lobeline was found to be absent in <i>L. chinensis</i>. All of the secondary metabolites in <i>L. chinensis</i> were determined with the HPLC/Q-TOF MS method. The absence of lobeline in <i>L. chinensis</i> was confirmed after this extensive study. |
topic |
<i>Lobelia chinensis</i> <i>Lobelia inflata</i> lobeline liquid chromatography mass spectrometry chemical profiling |
url |
https://www.mdpi.com/1420-3049/23/12/3258 |
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