Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand
Picralima nitida is used traditionally for management of cough. This study, therefore, investigated the antitussive, expectorant, and analgesic properties of the ethanolic seed extract of Picralima nitida (PNE), and ascertained its safety for use. Presence of secondary metabolites, and safety of PNE...
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doaj-d468e2118f39485eb6d60224904529e12021-08-02T11:41:40ZengWolters Kluwer Medknow PublicationsResearch in Pharmaceutical Sciences1735-53621735-94142016-01-01112100112Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. DurandGabriel DapaahGeorge Asumeng KoffuorPriscilla Kolibea ManteInemesit Okon BenPicralima nitida is used traditionally for management of cough. This study, therefore, investigated the antitussive, expectorant, and analgesic properties of the ethanolic seed extract of Picralima nitida (PNE), and ascertained its safety for use. Presence of secondary metabolites, and safety of PNE (10-2000 mg/kg) were evaluated by preliminary phytochemical screening, and by Irwin′s test respectively. Percentage reduction in cough count, percentage increase in latency of cough, and percentage protection offered by PNE were established by the citric acid-induced cough, acetylcholine- and Histamine-induced bronchoconstriction models. Dunkin-Hartley guinea pigs were treated with 100-500 mg/kg PNE or reference drugs, dihydrocodiene, atropine, mepyramine. Expectorant property of PNE (100-1000 mg/kg) was determined using the tracheal phenol red secretion; with ammonium chloride as a reference medication. Percentage maximal possible analgesic effect in the tail immersion test and the total nociceptive score in acetic acid-induced abdominal writhes, after treatment of BALB/c mice with PNE (100-500 mg/kg), diclofenac, and morphine were also estimated. Phytochemical screening revealed the presence of tannins, alkaloids, glycosides, saponins, steroids, terpenoids and anthraquinones. PNEdid not cause any extract-related physical, pharmacological and CNS toxicities or mortality; sedation was observed at doses 1000-2000 mg/kg. It showed significant dose-dependent reduction in cough count, and increased cough latency. PNE (1000 mg/kg) enhanced tracheal phenol red secretion. PNE (100-500 mg/kg) significantly and dose dependently increased tail withdrawal latencies, and nociceptive score. PNE has antitussive, expectorant, and analgesic properties, with an LD 50 >2000 mg/kg.http://www.jrps.ir/article.asp?issn=1735-5362;year=2016;volume=11;issue=2;spage=100;epage=112;aulast=DapaahTracheal phenol red secretion; Citric acid-induced cough; Total nociceptive score; Tail withdrawal latency; Picralima nitida |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gabriel Dapaah George Asumeng Koffuor Priscilla Kolibea Mante Inemesit Okon Ben |
spellingShingle |
Gabriel Dapaah George Asumeng Koffuor Priscilla Kolibea Mante Inemesit Okon Ben Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand Research in Pharmaceutical Sciences Tracheal phenol red secretion; Citric acid-induced cough; Total nociceptive score; Tail withdrawal latency; Picralima nitida |
author_facet |
Gabriel Dapaah George Asumeng Koffuor Priscilla Kolibea Mante Inemesit Okon Ben |
author_sort |
Gabriel Dapaah |
title |
Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand |
title_short |
Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand |
title_full |
Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand |
title_fullStr |
Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand |
title_full_unstemmed |
Antitussive, expectorant and analgesic effects of the ethanol seed extract of Picralima nitida (Stapf) Th. & H. Durand |
title_sort |
antitussive, expectorant and analgesic effects of the ethanol seed extract of picralima nitida (stapf) th. & h. durand |
publisher |
Wolters Kluwer Medknow Publications |
series |
Research in Pharmaceutical Sciences |
issn |
1735-5362 1735-9414 |
publishDate |
2016-01-01 |
description |
Picralima nitida is used traditionally for management of cough. This study, therefore, investigated the antitussive, expectorant, and analgesic properties of the ethanolic seed extract of Picralima nitida (PNE), and ascertained its safety for use. Presence of secondary metabolites, and safety of PNE (10-2000 mg/kg) were evaluated by preliminary phytochemical screening, and by Irwin′s test respectively. Percentage reduction in cough count, percentage increase in latency of cough, and percentage protection offered by PNE were established by the citric acid-induced cough, acetylcholine- and Histamine-induced bronchoconstriction models. Dunkin-Hartley guinea pigs were treated with 100-500 mg/kg PNE or reference drugs, dihydrocodiene, atropine, mepyramine. Expectorant property of PNE (100-1000 mg/kg) was determined using the tracheal phenol red secretion; with ammonium chloride as a reference medication. Percentage maximal possible analgesic effect in the tail immersion test and the total nociceptive score in acetic acid-induced abdominal writhes, after treatment of BALB/c mice with PNE (100-500 mg/kg), diclofenac, and morphine were also estimated. Phytochemical screening revealed the presence of tannins, alkaloids, glycosides, saponins, steroids, terpenoids and anthraquinones. PNEdid not cause any extract-related physical, pharmacological and CNS toxicities or mortality; sedation was observed at doses 1000-2000 mg/kg. It showed significant dose-dependent reduction in cough count, and increased cough latency. PNE (1000 mg/kg) enhanced tracheal phenol red secretion. PNE (100-500 mg/kg) significantly and dose dependently increased tail withdrawal latencies, and nociceptive score. PNE has antitussive, expectorant, and analgesic properties, with an LD 50 >2000 mg/kg. |
topic |
Tracheal phenol red secretion; Citric acid-induced cough; Total nociceptive score; Tail withdrawal latency; Picralima nitida |
url |
http://www.jrps.ir/article.asp?issn=1735-5362;year=2016;volume=11;issue=2;spage=100;epage=112;aulast=Dapaah |
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