Isolation and characterization of a dominant dwarf gene, d-h, in rice.
Plant height is an important agronomic trait that affects grain yield. Previously, we reported a novel semi-dominant dwarf mutant, HD1, derived from chemical mutagenesis using N-methyl-N-nitrosourea (MNU) on a japonica rice cultivar, Hwacheong. In this study, we cloned the gene responsible for the d...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3911911?pdf=render |
id |
doaj-d46a6f04f4c74dc0b685034c1e63d2f8 |
---|---|
record_format |
Article |
spelling |
doaj-d46a6f04f4c74dc0b685034c1e63d2f82020-11-25T01:09:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8621010.1371/journal.pone.0086210Isolation and characterization of a dominant dwarf gene, d-h, in rice.Rihua PiaoSang-Ho ChuWenzhu JiangYoye YuYongmei JinMi-Ok WooJoohyun LeeSunghan KimHee-Jong KohPlant height is an important agronomic trait that affects grain yield. Previously, we reported a novel semi-dominant dwarf mutant, HD1, derived from chemical mutagenesis using N-methyl-N-nitrosourea (MNU) on a japonica rice cultivar, Hwacheong. In this study, we cloned the gene responsible for the dwarf mutant using a map-based approach. Fine mapping revealed that the mutant gene was located on the short arm of chromosome 1 in a 48 kb region. Sequencing of the candidate genes and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) analysis identified the gene, d-h, which encodes a protein of unknown function but whose sequence is conserved in other cereal crops. Real-time (RT)-PCR analysis and promoter activity assays showed that the d-h gene was primarily expressed in the nodes and the panicle. In the HD1 plant, the d-h gene was found to carry a 63-bp deletion in the ORF region that was subsequently confirmed by transgenic experiments to be directly responsible for the gain-of-function phenotype observed in the mutant. Since the mutant plants exhibit a defect in GA response, but not in the GA synthetic pathway, it appears that the d-h gene may be involved in a GA signaling pathway.http://europepmc.org/articles/PMC3911911?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rihua Piao Sang-Ho Chu Wenzhu Jiang Yoye Yu Yongmei Jin Mi-Ok Woo Joohyun Lee Sunghan Kim Hee-Jong Koh |
spellingShingle |
Rihua Piao Sang-Ho Chu Wenzhu Jiang Yoye Yu Yongmei Jin Mi-Ok Woo Joohyun Lee Sunghan Kim Hee-Jong Koh Isolation and characterization of a dominant dwarf gene, d-h, in rice. PLoS ONE |
author_facet |
Rihua Piao Sang-Ho Chu Wenzhu Jiang Yoye Yu Yongmei Jin Mi-Ok Woo Joohyun Lee Sunghan Kim Hee-Jong Koh |
author_sort |
Rihua Piao |
title |
Isolation and characterization of a dominant dwarf gene, d-h, in rice. |
title_short |
Isolation and characterization of a dominant dwarf gene, d-h, in rice. |
title_full |
Isolation and characterization of a dominant dwarf gene, d-h, in rice. |
title_fullStr |
Isolation and characterization of a dominant dwarf gene, d-h, in rice. |
title_full_unstemmed |
Isolation and characterization of a dominant dwarf gene, d-h, in rice. |
title_sort |
isolation and characterization of a dominant dwarf gene, d-h, in rice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Plant height is an important agronomic trait that affects grain yield. Previously, we reported a novel semi-dominant dwarf mutant, HD1, derived from chemical mutagenesis using N-methyl-N-nitrosourea (MNU) on a japonica rice cultivar, Hwacheong. In this study, we cloned the gene responsible for the dwarf mutant using a map-based approach. Fine mapping revealed that the mutant gene was located on the short arm of chromosome 1 in a 48 kb region. Sequencing of the candidate genes and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) analysis identified the gene, d-h, which encodes a protein of unknown function but whose sequence is conserved in other cereal crops. Real-time (RT)-PCR analysis and promoter activity assays showed that the d-h gene was primarily expressed in the nodes and the panicle. In the HD1 plant, the d-h gene was found to carry a 63-bp deletion in the ORF region that was subsequently confirmed by transgenic experiments to be directly responsible for the gain-of-function phenotype observed in the mutant. Since the mutant plants exhibit a defect in GA response, but not in the GA synthetic pathway, it appears that the d-h gene may be involved in a GA signaling pathway. |
url |
http://europepmc.org/articles/PMC3911911?pdf=render |
work_keys_str_mv |
AT rihuapiao isolationandcharacterizationofadominantdwarfgenedhinrice AT sanghochu isolationandcharacterizationofadominantdwarfgenedhinrice AT wenzhujiang isolationandcharacterizationofadominantdwarfgenedhinrice AT yoyeyu isolationandcharacterizationofadominantdwarfgenedhinrice AT yongmeijin isolationandcharacterizationofadominantdwarfgenedhinrice AT miokwoo isolationandcharacterizationofadominantdwarfgenedhinrice AT joohyunlee isolationandcharacterizationofadominantdwarfgenedhinrice AT sunghankim isolationandcharacterizationofadominantdwarfgenedhinrice AT heejongkoh isolationandcharacterizationofadominantdwarfgenedhinrice |
_version_ |
1725178409963749376 |