Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

Summary: Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture,...

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Main Authors:	Anaïs Oudin, Virginie Baus, Vanessa Barthelemy, Carina Fabian, Eliane Klein, Monika Dieterle, May Wantz, Ann-Christin Hau, Claire Dording, Amandine Bernard, Alessandro Michelucci, Yahaya A. Yabo, Georgia Kanli, Olivier Keunen, Rolf Bjerkvig, Simone P. Niclou, Anna Golebiewska
Format:	Article
Language:	English
Published:	Elsevier 2021-06-01
Series:	STAR Protocols
Subjects:	Cell Biology Cell culture Cell isolation Cancer Model Organisms Neuroscience
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721002410

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record_format	Article
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Anaïs Oudin Virginie Baus Vanessa Barthelemy Carina Fabian Eliane Klein Monika Dieterle May Wantz Ann-Christin Hau Claire Dording Amandine Bernard Alessandro Michelucci Yahaya A. Yabo Georgia Kanli Olivier Keunen Rolf Bjerkvig Simone P. Niclou Anna Golebiewska
spellingShingle	Anaïs Oudin Virginie Baus Vanessa Barthelemy Carina Fabian Eliane Klein Monika Dieterle May Wantz Ann-Christin Hau Claire Dording Amandine Bernard Alessandro Michelucci Yahaya A. Yabo Georgia Kanli Olivier Keunen Rolf Bjerkvig Simone P. Niclou Anna Golebiewska Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors STAR Protocols Cell Biology Cell culture Cell isolation Cancer Model Organisms Neuroscience
author_facet	Anaïs Oudin Virginie Baus Vanessa Barthelemy Carina Fabian Eliane Klein Monika Dieterle May Wantz Ann-Christin Hau Claire Dording Amandine Bernard Alessandro Michelucci Yahaya A. Yabo Georgia Kanli Olivier Keunen Rolf Bjerkvig Simone P. Niclou Anna Golebiewska
author_sort	Anaïs Oudin
title	Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_short	Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_full	Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_fullStr	Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_full_unstemmed	Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
title_sort	protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-06-01
description	Summary: Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing.For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).
topic	Cell Biology Cell culture Cell isolation Cancer Model Organisms Neuroscience
url	http://www.sciencedirect.com/science/article/pii/S2666166721002410
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spelling	doaj-d48e3f8aa30341a5a0bf842b4b9702ff2021-06-21T04:25:35ZengElsevierSTAR Protocols2666-16672021-06-0122100534Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumorsAnaïs Oudin0Virginie Baus1Vanessa Barthelemy2Carina Fabian3Eliane Klein4Monika Dieterle5May Wantz6Ann-Christin Hau7Claire Dording8Amandine Bernard9Alessandro Michelucci10Yahaya A. Yabo11Georgia Kanli12Olivier Keunen13Rolf Bjerkvig14Simone P. Niclou15Anna Golebiewska16NORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Corresponding authorNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Department of Biomedicine, University of Bergen, 5019 Bergen, NorwayNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Neuro-Immunology Group, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 4367 Belvaux, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Quantitative Biology Unit, Luxembourg Institute of Health, 1445 Strassen, Luxembourg; Translational Radiomics, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Quantitative Biology Unit, Luxembourg Institute of Health, 1445 Strassen, Luxembourg; Translational Radiomics, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, LuxembourgNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Department of Biomedicine, University of Bergen, 5019 Bergen, NorwayNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Department of Biomedicine, University of Bergen, 5019 Bergen, NorwayNORLUX Neuro-Oncology Laboratory, Department of Oncology, Luxembourg Institute of Health, 1526 Luxembourg, Luxembourg; Corresponding authorSummary: Tumor organoids and patient-derived orthotopic xenografts (PDOXs) are some of the most valuable pre-clinical tools in cancer research. In this protocol, we describe efficient derivation of organoids and PDOX models from glioma patient tumors. We provide detailed steps for organoid culture, intracranial implantation, and detection of tumors in the brain. We further present technical adjustments for standardized functional assays and drug testing.For complete details on the use and execution of this protocol, please refer to Golebiewska et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721002410Cell BiologyCell cultureCell isolationCancerModel OrganismsNeuroscience

Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors

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