CIGAR‐seq, a CRISPR/Cas‐based method for unbiased screening of novel mRNA modification regulators

CIGAR‐seq, a CRISPR/Cas‐based method for unbiased screening of novel mRNA modification regulators

Abstract Cellular RNA is decorated with over 170 types of chemical modifications. Many modifications in mRNA, including m6A and m5C, have been associated with critical cellular functions under physiological and/or pathological conditions. To understand the biological functions of these modifications...

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Bibliographic Details
Main Authors: Liang Fang, Wen Wang, Guipeng Li, Li Zhang, Jun Li, Diwen Gan, Jiao Yang, Yisen Tang, Zewen Ding, Min Zhang, Wenhao Zhang, Daqi Deng, Zhengyu Song, Qionghua Zhu, Huanhuan Cui, Yuhui Hu, Wei Chen
Format: Article
Language:English
Published: Wiley 2020-11-01
Series:Molecular Systems Biology
Subjects:
CIGAR‐seq
m5C modification
mRNA modification
NSUN6
pooled CRISPR screen
Online Access:https://doi.org/10.15252/msb.202010025

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