The <it>CD14 </it>functional gene polymorphism -260 C>T is not involved in either the susceptibility to <it>Chlamydia trachomatis </it>infection or the development of tubal pathology

• Main Authors: Pleijster Jolein, Fennema Johan SA, Land Jolande A, den Hartog Janneke E, Spaargaren Joke, Ouburg Sander, Peña A Salvador, Morré Servaas A
• Format: Article
• Language: English
• Published: BMC 2005-12-01
• Series: BMC Infectious Diseases
• Online Access: http://www.biomedcentral.com/1471-2334/5/114

<p>Abstract</p> <p>Background</p> <p>The functional polymorphism -260 C>T in the LPS sensing TLR4 co-receptor <it>CD14 </it>gene enhances the transcriptional activity and results in a higher CD14 receptor density. Individuals carrying the T/T genotype also have significantly higher serum levels of soluble CD14. The T allele of this polymorphism has recently been linked to <it>Chlamydia pneumoniae </it>infection. We investigated the role of the <it>CD14 </it>-260 C>T polymorphism in the susceptibility to and severity (defined as subfertility and/or tubal pathology) of <it>C. trachomatis </it>infection in Dutch Caucasian women.</p> <p>Methods</p> <p>The different <it>CD14 </it>-260 C>T genotypes were assessed by PCR-based RFLP analysis in three cohorts: 1) A cohort (n = 576) of women attending a STD clinic, 2) a cohort (n = 253) of women with subfertility, and 3) an ethnically matched control cohort (n = 170). The following variables were used in the analysis: In cohort 1 the CT-DNA status, CT IgG serology status, self-reported symptoms and in cohort 2, the CT IgG serology status and the tubal status at laparoscopy.</p> <p>Results</p> <p>In the control cohort the CC, CT and TT genotype distribution was: 28.2%, 48.2%, and 23.5% respectively. No differences were found in the overall prevalence of <it>CD14 </it>-260 genotypes (28.1%, 50.7%, and 21.2%) in cohort 1 when compared to the control cohort. Also no differences were observed in women with or without CT-DNA, with or without serological CT responses, with or without symptoms, or in combinations of these three variables. In subfertile women with tubal pathology (cohort 2, n = 50) the genotype distribution was 28.0%, 48.0%, and 24.0% and in subfertile women without tubal pathology (n = 203), 27.6%, 49.3% and 23.2%. The genotype distribution was unchanged when CT IgG status was introduced in the analyses.</p> <p>Conclusion</p> <p>The <it>CD14 </it>-260 C>T genotype distributions were identical in all three cohorts, showing that this polymorphism is not involved in the susceptibility to or severity of sequelae of <it>C. trachomatis </it>infection.</p>