Detection of cyclohximide resistant gene in selected pathogenic fungi

• Main Authors: Gasak Falah Ali, Adnan H. Al-Hamadani, Adnan W Mohammad
• Format: Article
• Language: English
• Published: Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press 2016-01-01
• Series: Journal of Contemporary Medical Sciences
• Subjects: cycloheximide, cycloheximide resistant fungi, antifungal activity, cycloheximide resistance genes
• Online Access: http://jocms.org/index.php/jcms/article/view/48

Objective To detect the genes that play a role in resistance to cyclohximide by some pathogenic fungi. Methods Isolates of dermatophytic fungi (Trichophyton rubrum, Microsporum canis, Candida albicans and Aspergillus spp.) were isolated from patients with fungal infection attending the AL-Diwaniya Teaching Hospital and identified based on the microscopic and macroscopic characteristics. The effect of cycloheximide and ammonium hydroxide in isolation of these fungi in sabourauds dextrose agar were tested by the quantative transcrptive assay of gene expression (CYH1, CYH2, CDR1, CDR2 and ABC gene ) using real-time PCR. Results The result of this estimation revealed that the amplified DNA (PCR product) were 463 bp for CYH1; 265 bp for CDR1 ; 160 bp for CDR2; 359 bp for CYH2 in the pathogenic fungi C. albicans , T. rubrum, M. canis and A. niger. Conclusion The tested pathogens had resistance genes to cycloheximide such as (CHY1, CHY2, CDR1, CDR2 and HKG).