Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from b...

Full description

Bibliographic Details
Main Authors:	Nicolai T. Sand, Tobias B. Petersen, Sara R. Bang-Christensen, Theresa D. Ahrens, Caroline Løppke, Amalie M. Jørgensen, Tobias Gustavsson, Swati Choudhary, Thor G. Theander, Ali Salanti, Mette Ø. Agerbæk
Format:	Article
Language:	English
Published:	MDPI AG 2020-03-01
Series:	International Journal of Molecular Sciences
Subjects:	Circulating tumor cells (CTCs) rVAR2 enrichment technologies cancer diagnostics rare cell isolation
Online Access:	https://www.mdpi.com/1422-0067/21/7/2401

id	doaj-d505937b877846f59c0a0ee0d079a726
record_format	Article
spelling	doaj-d505937b877846f59c0a0ee0d079a7262020-11-25T02:41:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-03-01212401240110.3390/ijms21072401Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor CellsNicolai T. Sand0Tobias B. Petersen1Sara R. Bang-Christensen2Theresa D. Ahrens3Caroline Løppke4Amalie M. Jørgensen5Tobias Gustavsson6Swati Choudhary7Thor G. Theander8Ali Salanti9Mette Ø. Agerbæk10Centre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkVarCT Diagnostics, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen and Department of Infectious Diseases, Copenhagen University Hospital, 2200 Copenhagen, DenmarkEarly detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.https://www.mdpi.com/1422-0067/21/7/2401Circulating tumor cells (CTCs)rVAR2enrichment technologiescancerdiagnosticsrare cell isolation
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Nicolai T. Sand Tobias B. Petersen Sara R. Bang-Christensen Theresa D. Ahrens Caroline Løppke Amalie M. Jørgensen Tobias Gustavsson Swati Choudhary Thor G. Theander Ali Salanti Mette Ø. Agerbæk
spellingShingle	Nicolai T. Sand Tobias B. Petersen Sara R. Bang-Christensen Theresa D. Ahrens Caroline Løppke Amalie M. Jørgensen Tobias Gustavsson Swati Choudhary Thor G. Theander Ali Salanti Mette Ø. Agerbæk Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells International Journal of Molecular Sciences Circulating tumor cells (CTCs) rVAR2 enrichment technologies cancer diagnostics rare cell isolation
author_facet	Nicolai T. Sand Tobias B. Petersen Sara R. Bang-Christensen Theresa D. Ahrens Caroline Løppke Amalie M. Jørgensen Tobias Gustavsson Swati Choudhary Thor G. Theander Ali Salanti Mette Ø. Agerbæk
author_sort	Nicolai T. Sand
title	Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells
title_short	Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells
title_full	Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells
title_fullStr	Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells
title_full_unstemmed	Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells
title_sort	optimization of rvar2-based isolation of cancer cells in blood for building a robust assay for clinical detection of circulating tumor cells
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1661-6596 1422-0067
publishDate	2020-03-01
description	Early detection and monitoring of cancer progression is key to successful treatment. Therefore, much research is invested in developing technologies, enabling effective and valuable use of non-invasive liquid biopsies. This includes the detection and analysis of circulating tumor cells (CTCs) from blood samples. Recombinant malaria protein VAR2CSA (rVAR2) binds a unique chondroitin sulfate modification present on the vast majority of cancers and thereby holds promise as a near-universal tumor cell-targeting reagent to isolate CTCs from complex blood samples. This study describes a technical approach for optimizing the coupling of rVAR2 to magnetic beads and the development of a CTC isolation platform targeting a range of different cancer cell lines. We investigate both direct and indirect approaches for rVAR2-mediated bead retrieval of cancer cells and conclude that an indirect capture approach is most effective for rVAR2-based cancer cell retrieval.
topic	Circulating tumor cells (CTCs) rVAR2 enrichment technologies cancer diagnostics rare cell isolation
url	https://www.mdpi.com/1422-0067/21/7/2401
work_keys_str_mv	AT nicolaitsand optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT tobiasbpetersen optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT sararbangchristensen optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT theresadahrens optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT carolineløppke optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT amaliemjørgensen optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT tobiasgustavsson optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT swatichoudhary optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT thorgtheander optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT alisalanti optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells AT metteøagerbæk optimizationofrvar2basedisolationofcancercellsinbloodforbuildingarobustassayforclinicaldetectionofcirculatingtumorcells
_version_	1724777967105605632

Optimization of rVAR2-Based Isolation of Cancer Cells in Blood for Building a Robust Assay for Clinical Detection of Circulating Tumor Cells

Similar Items