Rapid generation of hypomorphic mutations
Hypomorphic mutations are a valuable tool for analysing gene function, but current methods have difficult to predict effects on gene expression and are organism specific. Here the authors present a pan-species approach using variable polyA tracks to modulate expression of a target protein.
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2017-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms14112 |
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doaj-d5276f10700a4a45bc605bc24297890b2021-05-11T07:23:02ZengNature Publishing GroupNature Communications2041-17232017-01-018111610.1038/ncomms14112Rapid generation of hypomorphic mutationsLaura L. Arthur0Joyce J. Chung1Preetam Janakirama2Kathryn M. Keefer3Igor Kolotilin4Slavica Pavlovic-Djuranovic5Douglas L. Chalker6Vojislava Grbic7Rachel Green8Rima Menassa9Heather L. True10James B. Skeath11Sergej Djuranovic12Department of Cell Biology and Physiology, Washington University School of MedicineDepartment of Biology, Washington UniversityDepartment of Biology, The University of Western OntarioDepartment of Cell Biology and Physiology, Washington University School of MedicineScattered Gold Biotechnology Inc. 14 Denali TerraceDepartment of Cell Biology and Physiology, Washington University School of MedicineDepartment of Biology, Washington UniversityDepartment of Biology, The University of Western OntarioDepartment of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of MedicineScience and Technology Branch, Agriculture and Agri-Food CanadaDepartment of Cell Biology and Physiology, Washington University School of MedicineDepartment of Genetics, Washington University School of MedicineDepartment of Cell Biology and Physiology, Washington University School of MedicineHypomorphic mutations are a valuable tool for analysing gene function, but current methods have difficult to predict effects on gene expression and are organism specific. Here the authors present a pan-species approach using variable polyA tracks to modulate expression of a target protein.https://doi.org/10.1038/ncomms14112 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Laura L. Arthur Joyce J. Chung Preetam Janakirama Kathryn M. Keefer Igor Kolotilin Slavica Pavlovic-Djuranovic Douglas L. Chalker Vojislava Grbic Rachel Green Rima Menassa Heather L. True James B. Skeath Sergej Djuranovic |
| spellingShingle |
Laura L. Arthur Joyce J. Chung Preetam Janakirama Kathryn M. Keefer Igor Kolotilin Slavica Pavlovic-Djuranovic Douglas L. Chalker Vojislava Grbic Rachel Green Rima Menassa Heather L. True James B. Skeath Sergej Djuranovic Rapid generation of hypomorphic mutations Nature Communications |
| author_facet |
Laura L. Arthur Joyce J. Chung Preetam Janakirama Kathryn M. Keefer Igor Kolotilin Slavica Pavlovic-Djuranovic Douglas L. Chalker Vojislava Grbic Rachel Green Rima Menassa Heather L. True James B. Skeath Sergej Djuranovic |
| author_sort |
Laura L. Arthur |
| title |
Rapid generation of hypomorphic mutations |
| title_short |
Rapid generation of hypomorphic mutations |
| title_full |
Rapid generation of hypomorphic mutations |
| title_fullStr |
Rapid generation of hypomorphic mutations |
| title_full_unstemmed |
Rapid generation of hypomorphic mutations |
| title_sort |
rapid generation of hypomorphic mutations |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2017-01-01 |
| description |
Hypomorphic mutations are a valuable tool for analysing gene function, but current methods have difficult to predict effects on gene expression and are organism specific. Here the authors present a pan-species approach using variable polyA tracks to modulate expression of a target protein. |
| url |
https://doi.org/10.1038/ncomms14112 |
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