Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes
Halophytes and glycophytes exhibit clear differences in their tolerance to high levels of salinity. The genetic mechanisms underlying this differentiation, however, remain unclear. To unveil these mechanisms, we surveyed the evolution of salinity-tolerant gene families through comparative genomic an...
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doaj-d52e84691b3548de862b0b315ca9bf1f2021-04-22T04:44:20ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.649001649001Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and GlycophytesRui Guo0Long Zhao1Long Zhao2Kaijian Zhang3Huiying Lu4Nadeem Bhanbhro5Chunwu Yang6Key Laboratory of Dryland Agriculture, Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun, ChinaInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, ChinaBeijing Novogene Bioinformatics Technology Ltd., Beijing, ChinaKey Laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun, ChinaKey Laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun, ChinaKey Laboratory of Molecular Epigenetics of Ministry of Education (MOE), Northeast Normal University, Changchun, ChinaHalophytes and glycophytes exhibit clear differences in their tolerance to high levels of salinity. The genetic mechanisms underlying this differentiation, however, remain unclear. To unveil these mechanisms, we surveyed the evolution of salinity-tolerant gene families through comparative genomic analyses between the model halophyte Puccinellia tenuiflora and glycophytic Gramineae plants, and compared their transcriptional and physiological responses to salinity stress. Under salinity stress, the K+ concentration in the root was slightly enhanced in P. tenuiflora, but it was greatly reduced in the glycophytic Gramineae plants, which provided a physiological explanation for differences in salinity tolerance between P. tenuiflora and these glycophytes. Interestingly, several K+ uptake gene families from P. tenuiflora experienced family expansion and positive selection during evolutionary history. This gene family expansion and the elevated expression of K+ uptake genes accelerated K+ accumulation and decreased Na+ toxicity in P. tenuiflora roots under salinity stress. Positively selected P. tenuiflora K+ uptake genes may have evolved new functions that contributed to development of P. tenuiflora salinity tolerance. In addition, the expansion of the gene families involved in pentose phosphate pathway, sucrose biosynthesis, and flavonoid biosynthesis assisted the adaptation of P. tenuiflora to survival under high salinity conditions.https://www.frontiersin.org/articles/10.3389/fpls.2021.649001/fullgene family expansiongenomehalophytePuccinellia tenuifloraRNA-seqpositive selection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rui Guo Long Zhao Long Zhao Kaijian Zhang Huiying Lu Nadeem Bhanbhro Chunwu Yang |
spellingShingle |
Rui Guo Long Zhao Long Zhao Kaijian Zhang Huiying Lu Nadeem Bhanbhro Chunwu Yang Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes Frontiers in Plant Science gene family expansion genome halophyte Puccinellia tenuiflora RNA-seq positive selection |
author_facet |
Rui Guo Long Zhao Long Zhao Kaijian Zhang Huiying Lu Nadeem Bhanbhro Chunwu Yang |
author_sort |
Rui Guo |
title |
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes |
title_short |
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes |
title_full |
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes |
title_fullStr |
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes |
title_full_unstemmed |
Comparative Genomics and Transcriptomics of the Extreme Halophyte Puccinellia tenuiflora Provides Insights Into Salinity Tolerance Differentiation Between Halophytes and Glycophytes |
title_sort |
comparative genomics and transcriptomics of the extreme halophyte puccinellia tenuiflora provides insights into salinity tolerance differentiation between halophytes and glycophytes |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-04-01 |
description |
Halophytes and glycophytes exhibit clear differences in their tolerance to high levels of salinity. The genetic mechanisms underlying this differentiation, however, remain unclear. To unveil these mechanisms, we surveyed the evolution of salinity-tolerant gene families through comparative genomic analyses between the model halophyte Puccinellia tenuiflora and glycophytic Gramineae plants, and compared their transcriptional and physiological responses to salinity stress. Under salinity stress, the K+ concentration in the root was slightly enhanced in P. tenuiflora, but it was greatly reduced in the glycophytic Gramineae plants, which provided a physiological explanation for differences in salinity tolerance between P. tenuiflora and these glycophytes. Interestingly, several K+ uptake gene families from P. tenuiflora experienced family expansion and positive selection during evolutionary history. This gene family expansion and the elevated expression of K+ uptake genes accelerated K+ accumulation and decreased Na+ toxicity in P. tenuiflora roots under salinity stress. Positively selected P. tenuiflora K+ uptake genes may have evolved new functions that contributed to development of P. tenuiflora salinity tolerance. In addition, the expansion of the gene families involved in pentose phosphate pathway, sucrose biosynthesis, and flavonoid biosynthesis assisted the adaptation of P. tenuiflora to survival under high salinity conditions. |
topic |
gene family expansion genome halophyte Puccinellia tenuiflora RNA-seq positive selection |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.649001/full |
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