Optimizing the production of suspension cells using the G-Rex “M” series
Broader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that...
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doaj-d53190c4394a4fa6b235fead976601632020-11-24T21:30:50ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012014-01-011C10.1038/mtm.2014.15Optimizing the production of suspension cells using the G-Rex “M” seriesPradip Bajgain0Roopa Mucharla1John Wilson2Dan Welch3Usanarat Anurathapan4Bitao Liang5Xiaohua Lu6Kyle Ripple7John M Centanni8Christine Hall9David Hsu10Larry A Couture11Shubhranshu Gupta12Adrian P Gee13Helen E Heslop14Ann M Leen15Cliona M Rooney16Juan F Vera17Center for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USAWilson Wolf Manufacturing Corporation, New Brighton, Minnesota, USAWilson Wolf Manufacturing Corporation, New Brighton, Minnesota, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACelgene Cellular Therapeutics, Celgene Corporation, Warren, New Jersey, USACelgene Cellular Therapeutics, Celgene Corporation, Warren, New Jersey, USAUniversity of Wisconsin–Madison, Waisman Biomanufacturing, Madison, Wisconsin, USAUniversity of Wisconsin–Madison, Waisman Biomanufacturing, Madison, Wisconsin, USACenter for Applied Technology Development, Beckman Research Institute of City of Hope, Duarte, California, USACenter for Applied Technology Development, Beckman Research Institute of City of Hope, Duarte, California, USACenter for Applied Technology Development, Beckman Research Institute of City of Hope, Duarte, California, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston Methodist Hospital, Texas Children's Hospital, Houston, Texas, USABroader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that supports large media volumes without compromising gas exchange. Although this culture platform has recently gained traction with the scientific community due to its superior performance when compared with traditional culture systems, the limits of this technology have yet to be explored. In this study, we investigated multiple variables including optimal seeding density and media volume, as well as maximum cell output per unit of surface area. Additionally, we have identified a novel means of estimating culture growth kinetics. All of these parameters were subsequently integrated into a novel G-Rex “M” series, which can accommodate these optimal conditions. A multicenter study confirmed that this fully optimized cell culture system can reliably produce a 100-fold cell expansion in only 10 days using 1L of medium. The G-Rex M series is linearly scalable and adaptable as a closed system, allowing an easy translation of preclinical protocols into the good manufacturing practice.http://www.sciencedirect.com/science/article/pii/S2329050116300808 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pradip Bajgain Roopa Mucharla John Wilson Dan Welch Usanarat Anurathapan Bitao Liang Xiaohua Lu Kyle Ripple John M Centanni Christine Hall David Hsu Larry A Couture Shubhranshu Gupta Adrian P Gee Helen E Heslop Ann M Leen Cliona M Rooney Juan F Vera |
spellingShingle |
Pradip Bajgain Roopa Mucharla John Wilson Dan Welch Usanarat Anurathapan Bitao Liang Xiaohua Lu Kyle Ripple John M Centanni Christine Hall David Hsu Larry A Couture Shubhranshu Gupta Adrian P Gee Helen E Heslop Ann M Leen Cliona M Rooney Juan F Vera Optimizing the production of suspension cells using the G-Rex “M” series Molecular Therapy: Methods & Clinical Development |
author_facet |
Pradip Bajgain Roopa Mucharla John Wilson Dan Welch Usanarat Anurathapan Bitao Liang Xiaohua Lu Kyle Ripple John M Centanni Christine Hall David Hsu Larry A Couture Shubhranshu Gupta Adrian P Gee Helen E Heslop Ann M Leen Cliona M Rooney Juan F Vera |
author_sort |
Pradip Bajgain |
title |
Optimizing the production of suspension cells using the G-Rex “M” series |
title_short |
Optimizing the production of suspension cells using the G-Rex “M” series |
title_full |
Optimizing the production of suspension cells using the G-Rex “M” series |
title_fullStr |
Optimizing the production of suspension cells using the G-Rex “M” series |
title_full_unstemmed |
Optimizing the production of suspension cells using the G-Rex “M” series |
title_sort |
optimizing the production of suspension cells using the g-rex “m” series |
publisher |
Elsevier |
series |
Molecular Therapy: Methods & Clinical Development |
issn |
2329-0501 |
publishDate |
2014-01-01 |
description |
Broader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that supports large media volumes without compromising gas exchange. Although this culture platform has recently gained traction with the scientific community due to its superior performance when compared with traditional culture systems, the limits of this technology have yet to be explored. In this study, we investigated multiple variables including optimal seeding density and media volume, as well as maximum cell output per unit of surface area. Additionally, we have identified a novel means of estimating culture growth kinetics. All of these parameters were subsequently integrated into a novel G-Rex “M” series, which can accommodate these optimal conditions. A multicenter study confirmed that this fully optimized cell culture system can reliably produce a 100-fold cell expansion in only 10 days using 1L of medium. The G-Rex M series is linearly scalable and adaptable as a closed system, allowing an easy translation of preclinical protocols into the good manufacturing practice. |
url |
http://www.sciencedirect.com/science/article/pii/S2329050116300808 |
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