The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat
Background & aim: Due to increased public interest in the use of herbal medicine, their efficacy and safety evaluation have become important. In some studies, Pistacia atlantica leave (PAL) extract suggested as a hypoglycemic agent. However, there is no study about its effects on diabetic nephro...
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Yasuj University Of Medical Sciences
2016-08-01
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doaj-d58244491b9640029c382826f677c0d22020-11-24T22:33:44ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65141728-65062016-08-01215420434The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in RatKH Vazifeshenas-Darmiyan0M Hosseini1R Rezaei2S Ezi3M Malekaneh4 Department of biochemistry, Birjand University of Medical Sciences, Birjand, Iran Research Centre of Experimental Medicine, Birjand University of Medical Sciences, Birjand, Iran, Department of biochemistry, Birjand University of Medical Sciences, Birjand, Iran Department of Anatomical Sciences, Birjand University of Medical Sciences,Birjand , Iran. Department of biochemistry, Birjand University of Medical Sciences, Birjand, Iran Background & aim: Due to increased public interest in the use of herbal medicine, their efficacy and safety evaluation have become important. In some studies, Pistacia atlantica leave (PAL) extract suggested as a hypoglycemic agent. However, there is no study about its effects on diabetic nephropathy. Therefore, the present work was conducted to evaluate the effects of aqueous extract of PAL on some biochemical and pathological aspects related to renal function in diabetic rats. Methods: In the present experimental study, 40 severe diabetes male Wistar rats (180-200 gr) with STZ and eight healthy rats at the same age were equally divided into 6 groups and followed for a period of thirty days as the oral treatment respectively. Diabetic groups I ,II and III received 100, 200 and 400 mg/kg of PAL extracts, diabetic group IV received 50 mg/kg Captopril and diabetic group V and healthy group received saline 0.9% as control groups. Finally, fasting blood glucose (FBG), Urine volume and 24 h urine total protein (UTP), blood nitrogen urea (BUN) and plasma creatinine (Cr) were assessed biochemically and also qualitative renal histomorphological alterations were assessed pathologically. Data were analyzed using one-way ANOVA and Tukey post hoc test. Results: In diabetic control group the FBS (383.5 ± 118.33), BUN (27.17± 2.86), Cr (1.3 ± 0.45), Urine volume (24.66± 5.10) and UTP (25.67 ± 1.51) of groups 1-5 significantly increased in comparison whit normal control group (p<0.0001 each). In addition: severe glomerolosclerosis, mesangial matrix expansion and tuft-capsule adhesion were observed in diabetic rats. Unlike captopril, which could significantly reduce the mean level of urea nitrogen (p <0.001; 12.5 ± 2.81), creatinine (p=0.034; 0.97±0.14) and protein (p=0.002: 13.66 ± 3.48) compared with diabetic controls the histology of the group was evaluate and confirmed its moderator effects, Conclusion: The results clearly demonstrate that PAL did not have any beneficial effects on diabetic nephropathy in rats.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-533-1&slc_lang=en&sid=1Pistacia atlantica Captopril Diabetic Nephropathy Rat |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
KH Vazifeshenas-Darmiyan M Hosseini R Rezaei S Ezi M Malekaneh |
spellingShingle |
KH Vazifeshenas-Darmiyan M Hosseini R Rezaei S Ezi M Malekaneh The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat Armaghane Danesh Bimonthly Journal Pistacia atlantica Captopril Diabetic Nephropathy Rat |
author_facet |
KH Vazifeshenas-Darmiyan M Hosseini R Rezaei S Ezi M Malekaneh |
author_sort |
KH Vazifeshenas-Darmiyan |
title |
The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat |
title_short |
The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat |
title_full |
The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat |
title_fullStr |
The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat |
title_full_unstemmed |
The Effects of Aqueous Extract of Wild Pistachio (Pistacia Atlantica) leaves on Diabetic Nephropathy in Rat |
title_sort |
effects of aqueous extract of wild pistachio (pistacia atlantica) leaves on diabetic nephropathy in rat |
publisher |
Yasuj University Of Medical Sciences |
series |
Armaghane Danesh Bimonthly Journal |
issn |
1728-6514 1728-6506 |
publishDate |
2016-08-01 |
description |
Background & aim: Due to increased public interest in the use of herbal medicine, their efficacy and safety evaluation have become important. In some studies, Pistacia atlantica leave (PAL) extract suggested as a hypoglycemic agent. However, there is no study about its effects on diabetic nephropathy. Therefore, the present work was conducted to evaluate the effects of aqueous extract of PAL on some biochemical and pathological aspects related to renal function in diabetic rats.
Methods: In the present experimental study, 40 severe diabetes male Wistar rats (180-200 gr) with STZ and eight healthy rats at the same age were equally divided into 6 groups and followed for a period of thirty days as the oral treatment respectively. Diabetic groups I ,II and III received 100, 200 and 400 mg/kg of PAL extracts, diabetic group IV received 50 mg/kg Captopril and diabetic group V and healthy group received saline 0.9% as control groups. Finally, fasting blood glucose (FBG), Urine volume and 24 h urine total protein (UTP), blood nitrogen urea (BUN) and plasma creatinine (Cr) were assessed biochemically and also qualitative renal histomorphological alterations were assessed pathologically. Data were analyzed using one-way ANOVA and Tukey post hoc test.
Results: In diabetic control group the FBS (383.5 ± 118.33), BUN (27.17± 2.86), Cr (1.3 ± 0.45), Urine volume (24.66± 5.10) and UTP (25.67 ± 1.51) of groups 1-5 significantly increased in comparison whit normal control group (p<0.0001 each). In addition: severe glomerolosclerosis, mesangial matrix expansion and tuft-capsule adhesion were observed in diabetic rats. Unlike captopril, which could significantly reduce the mean level of urea nitrogen (p <0.001; 12.5 ± 2.81), creatinine (p=0.034; 0.97±0.14) and protein (p=0.002: 13.66 ± 3.48) compared with diabetic controls the histology of the group was evaluate and confirmed its moderator effects,
Conclusion: The results clearly demonstrate that PAL did not have any beneficial effects on diabetic nephropathy in rats. |
topic |
Pistacia atlantica Captopril Diabetic Nephropathy Rat |
url |
http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-533-1&slc_lang=en&sid=1 |
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