Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia
Based on the cDNA sequences from hyper variable (HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase cDNA probes were designed, and a cDNA microarray for S-RNase detections was established. Each microarray contained 240 sites from 55 cDNA probes, including all specific cDNA se...
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KeAi Communications Co., Ltd.
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doaj-d5846353ad5e47c19e9095fb1f9f862c2021-04-02T11:52:29ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412015-11-011312113010.16420/j.issn.2095-9885.2016-0019Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifoliaJiang Nan0Tan Xiaofeng1Zhang Lin2Zhang Jingguo3Hu Hongju4School of Packing and Material Engineering, Hunan University of Technology, Zhuzhou, Hunan 412007, ChinaKey Laboratory of Cultivation and Protection for Non-wood Forest of Ministry of Education, the Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-south University of Forestry and Technology, Changsha 410004, ChinaKey Laboratory of Cultivation and Protection for Non-wood Forest of Ministry of Education, the Key Laboratory of Non-wood Forest Product of Forestry Ministry, Central-south University of Forestry and Technology, Changsha 410004, ChinaInstitute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaInstitute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaBased on the cDNA sequences from hyper variable (HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase cDNA probes were designed, and a cDNA microarray for S-RNase detections was established. Each microarray contained 240 sites from 55 cDNA probes, including all specific cDNA sequences from the HV regions of the S-alleles. Using the cDNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeling primers by PCR amplification, microarray hybridization detected the S-genotype of each pear cultivar. The genotypes inferred from the cDNA microarray hybridization signals of pear cultivars such as ‘Lijiang Huangsuanli’, ‘Xiuyu’, ‘Midu Yuli’, ‘Baimianli’, and ‘Deshengxiang’ were similar to the known genotypes of all tested cultivars. The S-RNase cDNA microarrays and the oligonucleotide gene chips were then used to conduct parallel testing of 24 P. pyrifolia cultivars with unknown S-genotypes. In conclusion, the construction of cDNA microarrays has further improved the pear S-RNase detection platform.http://www.sciencedirect.com/science/article/pii/S2468014115010183pearself-incompatibilityS-genotypecDNA microarrayoligonucleotide gene chip |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jiang Nan Tan Xiaofeng Zhang Lin Zhang Jingguo Hu Hongju |
spellingShingle |
Jiang Nan Tan Xiaofeng Zhang Lin Zhang Jingguo Hu Hongju Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia Horticultural Plant Journal pear self-incompatibility S-genotype cDNA microarray oligonucleotide gene chip |
author_facet |
Jiang Nan Tan Xiaofeng Zhang Lin Zhang Jingguo Hu Hongju |
author_sort |
Jiang Nan |
title |
Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia |
title_short |
Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia |
title_full |
Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia |
title_fullStr |
Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia |
title_full_unstemmed |
Establishment of Self-incompatibility Gene cDNA Microarray to Identify S-genotypes of Pyrus pyrifolia |
title_sort |
establishment of self-incompatibility gene cdna microarray to identify s-genotypes of pyrus pyrifolia |
publisher |
KeAi Communications Co., Ltd. |
series |
Horticultural Plant Journal |
issn |
2468-0141 |
publishDate |
2015-11-01 |
description |
Based on the cDNA sequences from hyper variable (HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase cDNA probes were designed, and a cDNA microarray for S-RNase detections was established. Each microarray contained 240 sites from 55 cDNA probes, including all specific cDNA sequences from the HV regions of the S-alleles. Using the cDNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeling primers by PCR amplification, microarray hybridization detected the S-genotype of each pear cultivar. The genotypes inferred from the cDNA microarray hybridization signals of pear cultivars such as ‘Lijiang Huangsuanli’, ‘Xiuyu’, ‘Midu Yuli’, ‘Baimianli’, and ‘Deshengxiang’ were similar to the known genotypes of all tested cultivars. The S-RNase cDNA microarrays and the oligonucleotide gene chips were then used to conduct parallel testing of 24 P. pyrifolia cultivars with unknown S-genotypes. In conclusion, the construction of cDNA microarrays has further improved the pear S-RNase detection platform. |
topic |
pear self-incompatibility S-genotype cDNA microarray oligonucleotide gene chip |
url |
http://www.sciencedirect.com/science/article/pii/S2468014115010183 |
work_keys_str_mv |
AT jiangnan establishmentofselfincompatibilitygenecdnamicroarraytoidentifysgenotypesofpyruspyrifolia AT tanxiaofeng establishmentofselfincompatibilitygenecdnamicroarraytoidentifysgenotypesofpyruspyrifolia AT zhanglin establishmentofselfincompatibilitygenecdnamicroarraytoidentifysgenotypesofpyruspyrifolia AT zhangjingguo establishmentofselfincompatibilitygenecdnamicroarraytoidentifysgenotypesofpyruspyrifolia AT huhongju establishmentofselfincompatibilitygenecdnamicroarraytoidentifysgenotypesofpyruspyrifolia |
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1721571106206253056 |