mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease
Aim: we aimed to construct a bioinformatics-based co-regulatory network of mRNAs and non coding RNAs (ncRNAs), which is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), followed by its validation in a NAFLD animal model. Materials and Methods: The mRNAs–miRNAs–lncRNAs reg...
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doaj-d5990b294ef24821ac4d557a27dcce172021-07-15T15:36:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-01226770677010.3390/ijms22136770mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver DiseaseMarwa Matboli0Shaimaa H. Gadallah1Wafaa M. Rashed2Amany Helmy Hasanin3Nada Essawy4Hala M. Ghanem5Sanaa Eissa6Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo 11382, EgyptDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, EgyptDepartment of Research, Children’s Cancer Hospital-57357, Cairo 11382, EgyptDepartment of Clinical Pharmacology, Faculty of Medicine, Ain Shams University, Cairo 11382, EgyptInstitut Pasteur, CEDEX 15, 75724 Paris, FranceDepartment of Biochemistry, Faculty of Science, Ain Shams University, Cairo 11382, EgyptDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Ain Shams University, Cairo 11382, EgyptAim: we aimed to construct a bioinformatics-based co-regulatory network of mRNAs and non coding RNAs (ncRNAs), which is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), followed by its validation in a NAFLD animal model. Materials and Methods: The mRNAs–miRNAs–lncRNAs regulatory network involved in NAFLD was retrieved and constructed utilizing bioinformatics tools. Then, we validated this network using an NAFLD animal model, high sucrose and high fat diet (HSHF)-fed rats. Finally, the expression level of the network players was assessed in the liver tissues using reverse transcriptase real-time polymerase chain reaction. Results: in-silico constructed network revealed six mRNAs (<i>YAP1, FOXA2, AMOTL2, TEAD2, SMAD4</i> and <i>NF2</i>), two miRNAs (<i>miR-650</i> and <i>miR-1205</i>), and two lncRNAs (RPARP-AS1 and <i>SRD5A3-AS1</i>) that play important roles as a co-regulatory network in NAFLD pathogenesis. Moreover, the expression level of these constructed network–players was significantly different between NAFLD and normal control. Conclusion and future perspectives: this study provides new insight into the molecular mechanism of NAFLD pathogenesis and valuable clues for the potential use of the constructed RNA network in effective diagnostic or management strategies of NAFLD.https://www.mdpi.com/1422-0067/22/13/6770NAFLDNon-Alcoholic SteatoHepatitis (NASH)Hippo signalingmiRNAslncRNAsncRNAs network |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marwa Matboli Shaimaa H. Gadallah Wafaa M. Rashed Amany Helmy Hasanin Nada Essawy Hala M. Ghanem Sanaa Eissa |
spellingShingle |
Marwa Matboli Shaimaa H. Gadallah Wafaa M. Rashed Amany Helmy Hasanin Nada Essawy Hala M. Ghanem Sanaa Eissa mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease International Journal of Molecular Sciences NAFLD Non-Alcoholic SteatoHepatitis (NASH) Hippo signaling miRNAs lncRNAs ncRNAs network |
author_facet |
Marwa Matboli Shaimaa H. Gadallah Wafaa M. Rashed Amany Helmy Hasanin Nada Essawy Hala M. Ghanem Sanaa Eissa |
author_sort |
Marwa Matboli |
title |
mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease |
title_short |
mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease |
title_full |
mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease |
title_fullStr |
mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease |
title_full_unstemmed |
mRNA-miRNA-lncRNA Regulatory Network in Nonalcoholic Fatty Liver Disease |
title_sort |
mrna-mirna-lncrna regulatory network in nonalcoholic fatty liver disease |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-06-01 |
description |
Aim: we aimed to construct a bioinformatics-based co-regulatory network of mRNAs and non coding RNAs (ncRNAs), which is implicated in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), followed by its validation in a NAFLD animal model. Materials and Methods: The mRNAs–miRNAs–lncRNAs regulatory network involved in NAFLD was retrieved and constructed utilizing bioinformatics tools. Then, we validated this network using an NAFLD animal model, high sucrose and high fat diet (HSHF)-fed rats. Finally, the expression level of the network players was assessed in the liver tissues using reverse transcriptase real-time polymerase chain reaction. Results: in-silico constructed network revealed six mRNAs (<i>YAP1, FOXA2, AMOTL2, TEAD2, SMAD4</i> and <i>NF2</i>), two miRNAs (<i>miR-650</i> and <i>miR-1205</i>), and two lncRNAs (RPARP-AS1 and <i>SRD5A3-AS1</i>) that play important roles as a co-regulatory network in NAFLD pathogenesis. Moreover, the expression level of these constructed network–players was significantly different between NAFLD and normal control. Conclusion and future perspectives: this study provides new insight into the molecular mechanism of NAFLD pathogenesis and valuable clues for the potential use of the constructed RNA network in effective diagnostic or management strategies of NAFLD. |
topic |
NAFLD Non-Alcoholic SteatoHepatitis (NASH) Hippo signaling miRNAs lncRNAs ncRNAs network |
url |
https://www.mdpi.com/1422-0067/22/13/6770 |
work_keys_str_mv |
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