Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland
Background: Livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza H1N1, rotavirus G8/G9, and MERS-CoV. Surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impac...
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doaj-d59d9eec8b5b4b4992b92fe412faf53c2020-11-25T01:06:50ZengTaylor & Francis GroupInfection Ecology & Epidemiology2000-86862015-06-015011110.3402/iee.v5.2727027270Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in IrelandLynda Gunn0Patrick James Collins1Séamus Fanning2John McKillen3John Morgan4Anthony Staines5Helen O'shea6 Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland School of Public Health, Physio & Pop Sc, Science Centre – South, Dublin 4, Ireland Veterinary Sciences Division, Agri-Food and Biosciences Institute, Belfast, United Kingdom School of Microbiology, University College Cork, Cork, Ireland School of Nursing, Dublin City University, Dublin 9, Ireland Department of Biological Sciences, Cork Institute of Technology, Cork, IrelandBackground: Livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza H1N1, rotavirus G8/G9, and MERS-CoV. Surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impact, for example, failure to thrive. There is a paucity of data regarding detection and characterisation of gastroenteritis viruses, particularly novel viruses, in porcines in Ireland. Recently, a number of small novel porcine DNA viruses have emerged globally, for example, torque teno sus virus, porcine bocavirus, and parvoviruses 2 & 4, and little is known about the biology and potential pathogenicity of these viruses. Bocaparvovirus is a genetically distinct group of viruses which has been recently detected in humans and animals. Methods: In this study, the presence of gastroenteritis viruses (rotavirus A, porcine circovirus, adenovirus, and porcine bocavirus) was investigated in a selection of archived faecal samples from asymptomatic piglets from a commercial farm in Ireland. A total of 104 specimens were pooled and screened using conventional molecular techniques (PCR and RT-PCR), a subset of specimens (n=44) were then examined individually. Viral diversity was then investigated using statistical and phylogenetic techniques. Results: Initial screening showed a high prevalence of PBoV in this farm, with the formation of three distinct groups in phylogenetic analysis. Other viruses were also investigated in this study with the first report of PCV, PAdV and lineage I G5 RVA in Ireland. Some specimens contained >1 virus, with statistical analysis indicating a strong correlation for mixed infections of PBoV and PAdV on this farm. Conclusion: Investigating the diversity of circulating enteric viruses on Irish porcine farms is important to improve the prophylactic tools available and to facilitate the early detection of changes in circulating viruses.http://www.infectionecologyandepidemiology.net/index.php/iee/article/view/27270/pdf_12bocavirusBocaparvovirusporcinegastroenteritis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lynda Gunn Patrick James Collins Séamus Fanning John McKillen John Morgan Anthony Staines Helen O'shea |
spellingShingle |
Lynda Gunn Patrick James Collins Séamus Fanning John McKillen John Morgan Anthony Staines Helen O'shea Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland Infection Ecology & Epidemiology bocavirus Bocaparvovirus porcine gastroenteritis |
author_facet |
Lynda Gunn Patrick James Collins Séamus Fanning John McKillen John Morgan Anthony Staines Helen O'shea |
author_sort |
Lynda Gunn |
title |
Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland |
title_short |
Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland |
title_full |
Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland |
title_fullStr |
Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland |
title_full_unstemmed |
Detection and characterisation of novel bocavirus (genus Bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in Ireland |
title_sort |
detection and characterisation of novel bocavirus (genus bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in ireland |
publisher |
Taylor & Francis Group |
series |
Infection Ecology & Epidemiology |
issn |
2000-8686 |
publishDate |
2015-06-01 |
description |
Background: Livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza H1N1, rotavirus G8/G9, and MERS-CoV. Surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impact, for example, failure to thrive. There is a paucity of data regarding detection and characterisation of gastroenteritis viruses, particularly novel viruses, in porcines in Ireland. Recently, a number of small novel porcine DNA viruses have emerged globally, for example, torque teno sus virus, porcine bocavirus, and parvoviruses 2 & 4, and little is known about the biology and potential pathogenicity of these viruses. Bocaparvovirus is a genetically distinct group of viruses which has been recently detected in humans and animals. Methods: In this study, the presence of gastroenteritis viruses (rotavirus A, porcine circovirus, adenovirus, and porcine bocavirus) was investigated in a selection of archived faecal samples from asymptomatic piglets from a commercial farm in Ireland. A total of 104 specimens were pooled and screened using conventional molecular techniques (PCR and RT-PCR), a subset of specimens (n=44) were then examined individually. Viral diversity was then investigated using statistical and phylogenetic techniques. Results: Initial screening showed a high prevalence of PBoV in this farm, with the formation of three distinct groups in phylogenetic analysis. Other viruses were also investigated in this study with the first report of PCV, PAdV and lineage I G5 RVA in Ireland. Some specimens contained >1 virus, with statistical analysis indicating a strong correlation for mixed infections of PBoV and PAdV on this farm. Conclusion: Investigating the diversity of circulating enteric viruses on Irish porcine farms is important to improve the prophylactic tools available and to facilitate the early detection of changes in circulating viruses. |
topic |
bocavirus Bocaparvovirus porcine gastroenteritis |
url |
http://www.infectionecologyandepidemiology.net/index.php/iee/article/view/27270/pdf_12 |
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