New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae<...
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doaj-d5b6ee5d100446d6a91ce36b97dcfa2e2021-01-06T00:00:27ZengMDPI AGCells2073-44092021-01-0110696910.3390/cells10010069New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular TrapMarie Chambard0Carole Plasson1Céline Derambure2Sophie Coutant3Isabelle Tournier4Benjamin Lefranc5Jérôme Leprince6Marie-Christine Kiefer-Meyer7Azeddine Driouich8Marie-Laure Follet-Gueye9Isabelle Boulogne10Normandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FrancePlateforme de Recherche en Imagerie Cellulaire de Normandie (PRIMACEN), Normandie Université UNIROUEN, INSERM U1239, 76000 Rouen, FrancePlateforme de Recherche en Imagerie Cellulaire de Normandie (PRIMACEN), Normandie Université UNIROUEN, INSERM U1239, 76000 Rouen, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceexDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae</i> with worldwide interest: soybean (<i>Glycine max</i> (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus <i>Phytophthora</i>). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.https://www.mdpi.com/2073-4409/10/1/69plant exDNAroot extracellular trap (RET)high-throughput DNA sequencingPEP-13<i>Glycine max</i> (L.) Merr |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marie Chambard Carole Plasson Céline Derambure Sophie Coutant Isabelle Tournier Benjamin Lefranc Jérôme Leprince Marie-Christine Kiefer-Meyer Azeddine Driouich Marie-Laure Follet-Gueye Isabelle Boulogne |
spellingShingle |
Marie Chambard Carole Plasson Céline Derambure Sophie Coutant Isabelle Tournier Benjamin Lefranc Jérôme Leprince Marie-Christine Kiefer-Meyer Azeddine Driouich Marie-Laure Follet-Gueye Isabelle Boulogne New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap Cells plant exDNA root extracellular trap (RET) high-throughput DNA sequencing PEP-13 <i>Glycine max</i> (L.) Merr |
author_facet |
Marie Chambard Carole Plasson Céline Derambure Sophie Coutant Isabelle Tournier Benjamin Lefranc Jérôme Leprince Marie-Christine Kiefer-Meyer Azeddine Driouich Marie-Laure Follet-Gueye Isabelle Boulogne |
author_sort |
Marie Chambard |
title |
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap |
title_short |
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap |
title_full |
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap |
title_fullStr |
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap |
title_full_unstemmed |
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap |
title_sort |
new insights into plant extracellular dna. a study in soybean root extracellular trap |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2021-01-01 |
description |
exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae</i> with worldwide interest: soybean (<i>Glycine max</i> (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus <i>Phytophthora</i>). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA. |
topic |
plant exDNA root extracellular trap (RET) high-throughput DNA sequencing PEP-13 <i>Glycine max</i> (L.) Merr |
url |
https://www.mdpi.com/2073-4409/10/1/69 |
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