New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap

New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap

exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae<...

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Main Authors: Marie Chambard, Carole Plasson, Céline Derambure, Sophie Coutant, Isabelle Tournier, Benjamin Lefranc, Jérôme Leprince, Marie-Christine Kiefer-Meyer, Azeddine Driouich, Marie-Laure Follet-Gueye, Isabelle Boulogne
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Cells
Subjects:
plant exDNA
root extracellular trap (RET)
high-throughput DNA sequencing
PEP-13
<i>Glycine max</i> (L.) Merr
Online Access:https://www.mdpi.com/2073-4409/10/1/69
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spelling doaj-d5b6ee5d100446d6a91ce36b97dcfa2e2021-01-06T00:00:27ZengMDPI AGCells2073-44092021-01-0110696910.3390/cells10010069New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular TrapMarie Chambard0Carole Plasson1Céline Derambure2Sophie Coutant3Isabelle Tournier4Benjamin Lefranc5Jérôme Leprince6Marie-Christine Kiefer-Meyer7Azeddine Driouich8Marie-Laure Follet-Gueye9Isabelle Boulogne10Normandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FranceNormandy Center for Genomic and Personalized Medicine, 76000 Rouen, FrancePlateforme de Recherche en Imagerie Cellulaire de Normandie (PRIMACEN), Normandie Université UNIROUEN, INSERM U1239, 76000 Rouen, FrancePlateforme de Recherche en Imagerie Cellulaire de Normandie (PRIMACEN), Normandie Université UNIROUEN, INSERM U1239, 76000 Rouen, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceNormandie University, UNIROUEN, UFR des Sciences et Techniques, Glyco-MEV EA4358, SFR NORVEGE FED 4277, 76821 Mont-Saint-Aignan, FranceexDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae</i> with worldwide interest: soybean (<i>Glycine max</i> (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus <i>Phytophthora</i>). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.https://www.mdpi.com/2073-4409/10/1/69plant exDNAroot extracellular trap (RET)high-throughput DNA sequencingPEP-13<i>Glycine max</i> (L.) Merr
collection DOAJ
language English
format Article
sources DOAJ
author Marie Chambard
Carole Plasson
Céline Derambure
Sophie Coutant
Isabelle Tournier
Benjamin Lefranc
Jérôme Leprince
Marie-Christine Kiefer-Meyer
Azeddine Driouich
Marie-Laure Follet-Gueye
Isabelle Boulogne
spellingShingle Marie Chambard
Carole Plasson
Céline Derambure
Sophie Coutant
Isabelle Tournier
Benjamin Lefranc
Jérôme Leprince
Marie-Christine Kiefer-Meyer
Azeddine Driouich
Marie-Laure Follet-Gueye
Isabelle Boulogne
New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
Cells
plant exDNA
root extracellular trap (RET)
high-throughput DNA sequencing
PEP-13
<i>Glycine max</i> (L.) Merr
author_facet Marie Chambard
Carole Plasson
Céline Derambure
Sophie Coutant
Isabelle Tournier
Benjamin Lefranc
Jérôme Leprince
Marie-Christine Kiefer-Meyer
Azeddine Driouich
Marie-Laure Follet-Gueye
Isabelle Boulogne
author_sort Marie Chambard
title New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
title_short New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
title_full New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
title_fullStr New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
title_full_unstemmed New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
title_sort new insights into plant extracellular dna. a study in soybean root extracellular trap
publisher MDPI AG
series Cells
issn 2073-4409
publishDate 2021-01-01
description exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a <i>Fabaceae</i> with worldwide interest: soybean (<i>Glycine max</i> (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus <i>Phytophthora</i>). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.
topic plant exDNA
root extracellular trap (RET)
high-throughput DNA sequencing
PEP-13
<i>Glycine max</i> (L.) Merr
url https://www.mdpi.com/2073-4409/10/1/69
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