Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey
Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresista...
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doaj-d5b73be751de427b85eb5be9a1a61c382020-11-24T23:40:08ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312012-06-01292160165Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western TurkeyHörü GaziAyşe ÖzsözCan BiçmenNuri ÖzkütükSelami GünalSüheyla SürücüoğluRıza DurmazObjective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results: The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions: The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains. http://balkanmedicaljournal.org/text.php3?id=854Mycobacterium tuberculosisdrug resistancegenotypingepidemiology |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hörü Gazi Ayşe Özsöz Can Biçmen Nuri Özkütük Selami Günal Süheyla Sürücüoğlu Rıza Durmaz |
spellingShingle |
Hörü Gazi Ayşe Özsöz Can Biçmen Nuri Özkütük Selami Günal Süheyla Sürücüoğlu Rıza Durmaz Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey Balkan Medical Journal Mycobacterium tuberculosis drug resistance genotyping epidemiology |
author_facet |
Hörü Gazi Ayşe Özsöz Can Biçmen Nuri Özkütük Selami Günal Süheyla Sürücüoğlu Rıza Durmaz |
author_sort |
Hörü Gazi |
title |
Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey |
title_short |
Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey |
title_full |
Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey |
title_fullStr |
Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey |
title_full_unstemmed |
Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey |
title_sort |
molecular diversity of drug resistant mycobacterium tuberculosis strains in western turkey |
publisher |
Galenos Publishing House |
series |
Balkan Medical Journal |
issn |
2146-3123 2146-3131 |
publishDate |
2012-06-01 |
description |
Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results: The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions: The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains. |
topic |
Mycobacterium tuberculosis drug resistance genotyping epidemiology |
url |
http://balkanmedicaljournal.org/text.php3?id=854 |
work_keys_str_mv |
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