Molecular Diversity of Drug Resistant Mycobacterium Tuberculosis Strains in Western Turkey

• Main Authors: Hörü Gazi, Ayşe Özsöz, Can Biçmen, Nuri Özkütük, Selami Günal, Süheyla Sürücüoğlu, Rıza Durmaz
• Format: Article
• Language: English
• Published: Galenos Publishing House 2012-06-01
• Series: Balkan Medical Journal
• Subjects: Mycobacterium tuberculosis; drug resistance; genotyping; epidemiology
• Online Access: http://balkanmedicaljournal.org/text.php3?id=854

Objective: The aim of this study was to investigate the molecular diversity and clonal relationship of drug resistant Mycobacterium tuberculosis strains isolated in Western Turkey. Materials and Methods: A total of 87 strains isolated between 2006 and 2009, eight of which were rifampicin monoresistant and 79 were multidrug resistant, were analyzed with IS6110 RFLP and spoligotyping methods. Results: The results of spoligotyping showed that 7% of the strains were orphans, and 8% were undefined for family in the SpolDB4 database. Major families of the strains were LAM (38%), T (35%), Haarlem (7%), Beijing (2%), S (2%) and U (1%) families. The clustering rate by spoligotyping was calculated as 75%. The most predominant SIT cluster was SIT41 (29%). According to the results of IS6110 RFLP, 71 different patterns of IS6110 were observed. Low copy number was found in 26% of the strains. When the results of two methods were combined, the final clustering rate was calculated as 26%. Conclusions: The genotypical distribution of drug resistant tuberculosis isolates in our region indicates genetic diversity and the clustering rate was found low in our region. However, more comprehensive and long-term molecular epidemiological studies are needed to control the drug resistant strains.