Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue

Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehens...

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Bibliographic Details
Main Authors: M. Sadman Sakib, Godwin Sokpor, Huu Phuc Nguyen, Andre Fischer, Tran Tuoc
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Flow Cytometry/Mass Cytometry
Genomics
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000253
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spelling doaj-d5bb025d2c1e41a18b96815dc426ee612021-03-22T12:53:12ZengElsevierSTAR Protocols2666-16672021-03-0121100318Intranuclear immunostaining-based FACS protocol from embryonic cortical tissueM. Sadman Sakib0Godwin Sokpor1Huu Phuc Nguyen2Andre Fischer3Tran Tuoc4German Center for Neurodegenerative Diseases, 37077 Goettingen, GermanyRuhr University of Bochum, 44791 Bochum, GermanyRuhr University of Bochum, 44791 Bochum, GermanyGerman Center for Neurodegenerative Diseases, 37077 Goettingen, Germany; Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells (MBExC),” 37077 Goettingen, Germany; Corresponding authorRuhr University of Bochum, 44791 Bochum, Germany; Corresponding authorSummary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.http://www.sciencedirect.com/science/article/pii/S2666166721000253Flow Cytometry/Mass CytometryGenomicsNeuroscience
collection DOAJ
language English
format Article
sources DOAJ
author M. Sadman Sakib
Godwin Sokpor
Huu Phuc Nguyen
Andre Fischer
Tran Tuoc
spellingShingle M. Sadman Sakib
Godwin Sokpor
Huu Phuc Nguyen
Andre Fischer
Tran Tuoc
Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
STAR Protocols
Flow Cytometry/Mass Cytometry
Genomics
Neuroscience
author_facet M. Sadman Sakib
Godwin Sokpor
Huu Phuc Nguyen
Andre Fischer
Tran Tuoc
author_sort M. Sadman Sakib
title Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
title_short Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
title_full Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
title_fullStr Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
title_full_unstemmed Intranuclear immunostaining-based FACS protocol from embryonic cortical tissue
title_sort intranuclear immunostaining-based facs protocol from embryonic cortical tissue
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Cell sorting can be used to purify cell populations for cell type-specific molecular probing. Fluorescence-activated cell sorting (FACS) coupled with high-throughput sequencing affords molecular signature identification for specific cell types. FACS has many challenges that limit comprehensive cell purification from the brain, leading to incomplete molecular characterization. Here, we present the intranuclear immunostaining-based FACS protocol with several modified steps, which allows optimized nuclei/cell sorting from mouse or human embryonic cortical tissue for distinct downstream molecular investigation of basal intermediate progenitors.
topic Flow Cytometry/Mass Cytometry
Genomics
Neuroscience
url http://www.sciencedirect.com/science/article/pii/S2666166721000253
work_keys_str_mv AT msadmansakib intranuclearimmunostainingbasedfacsprotocolfromembryoniccorticaltissue
AT godwinsokpor intranuclearimmunostainingbasedfacsprotocolfromembryoniccorticaltissue
AT huuphucnguyen intranuclearimmunostainingbasedfacsprotocolfromembryoniccorticaltissue
AT andrefischer intranuclearimmunostainingbasedfacsprotocolfromembryoniccorticaltissue
AT trantuoc intranuclearimmunostainingbasedfacsprotocolfromembryoniccorticaltissue
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