Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling
Background: Maintenance of brain circulation during shock is sufficient to prevent subcortical injury but the cerebral cortex is not spared. This suggests area-specific regulation of cerebral blood flow (CBF) during hemorrhage. Methods: Cortical and subcortical CBF were continuously measured during...
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doaj-d5cdd3ba062640fc9cc4d44528f4b7d02020-11-24T23:41:09ZengMDPI AGInternational Journal of Molecular Sciences1422-00672017-05-01185103110.3390/ijms18051031ijms18051031Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic SignalingRené Schiffner0Sabine Juliane Bischoff1Thomas Lehmann2Florian Rakers3Sven Rupprecht4Juliane Reiche5Georg Matziolis6Harald Schubert7Matthias Schwab8Otmar Huber9Martin Schmidt10Orthopedic Department, Campus Eisenberg, Jena University Hospital-Friedrich Schiller University, 07607 Eisenberg, GermanyInstitute for Laboratory Animal Sciences and Welfare, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyInstitute of Medical Statistics, Computer Sciences and Documentation Science, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyDepartment of Neurology; Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyDepartment of Neurology; Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyInstitute for Biochemistry II, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyOrthopedic Department, Campus Eisenberg, Jena University Hospital-Friedrich Schiller University, 07607 Eisenberg, GermanyInstitute for Laboratory Animal Sciences and Welfare, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyDepartment of Neurology; Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyInstitute for Biochemistry II, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyInstitute for Biochemistry II, Jena University Hospital-Friedrich Schiller University, 07743 Jena, GermanyBackground: Maintenance of brain circulation during shock is sufficient to prevent subcortical injury but the cerebral cortex is not spared. This suggests area-specific regulation of cerebral blood flow (CBF) during hemorrhage. Methods: Cortical and subcortical CBF were continuously measured during blood loss (≤50%) and subsequent reperfusion using laser Doppler flowmetry. Blood gases, mean arterial blood pressure (MABP), heart rate and renal blood flow were also monitored. Urapidil was used for α1A-adrenergic receptor blockade in dosages, which did not modify the MABP-response to blood loss. Western blot and quantitative reverse transcription polymerase chain reactions were used to determine adrenergic receptor expression in brain arterioles. Results: During hypovolemia subcortical CBF was maintained at 81 ± 6% of baseline, whereas cortical CBF decreased to 40 ± 4% (p < 0.001). Reperfusion led to peak CBFs of about 70% above baseline in both brain regions. α1A-Adrenergic blockade massively reduced subcortical CBF during hemorrhage and reperfusion, and prevented hyperperfusion during reperfusion in the cortex. α1A-mRNA expression was significantly higher in the cortex, whereas α1D-mRNA expression was higher in the subcortex (p < 0.001). Conclusions: α1-Adrenergic receptors are critical for perfusion redistribution: activity of the α1A-receptor subtype is a prerequisite for redistribution of CBF, whereas the α1D-receptor subtype may determine the magnitude of redistribution responses.http://www.mdpi.com/1422-0067/18/5/1031adrenergic regulationalpha-adrenergiccerebral blood flowcerebral hemodynamicsresuscitationhead traumacerebrovasvular diseaseneurodegenerative disease |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
René Schiffner Sabine Juliane Bischoff Thomas Lehmann Florian Rakers Sven Rupprecht Juliane Reiche Georg Matziolis Harald Schubert Matthias Schwab Otmar Huber Martin Schmidt |
spellingShingle |
René Schiffner Sabine Juliane Bischoff Thomas Lehmann Florian Rakers Sven Rupprecht Juliane Reiche Georg Matziolis Harald Schubert Matthias Schwab Otmar Huber Martin Schmidt Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling International Journal of Molecular Sciences adrenergic regulation alpha-adrenergic cerebral blood flow cerebral hemodynamics resuscitation head trauma cerebrovasvular disease neurodegenerative disease |
author_facet |
René Schiffner Sabine Juliane Bischoff Thomas Lehmann Florian Rakers Sven Rupprecht Juliane Reiche Georg Matziolis Harald Schubert Matthias Schwab Otmar Huber Martin Schmidt |
author_sort |
René Schiffner |
title |
Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling |
title_short |
Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling |
title_full |
Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling |
title_fullStr |
Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling |
title_full_unstemmed |
Redistribution of Cerebral Blood Flow during Severe Hypovolemia and Reperfusion in a Sheep Model: Critical Role of α1-Adrenergic Signaling |
title_sort |
redistribution of cerebral blood flow during severe hypovolemia and reperfusion in a sheep model: critical role of α1-adrenergic signaling |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2017-05-01 |
description |
Background: Maintenance of brain circulation during shock is sufficient to prevent subcortical injury but the cerebral cortex is not spared. This suggests area-specific regulation of cerebral blood flow (CBF) during hemorrhage. Methods: Cortical and subcortical CBF were continuously measured during blood loss (≤50%) and subsequent reperfusion using laser Doppler flowmetry. Blood gases, mean arterial blood pressure (MABP), heart rate and renal blood flow were also monitored. Urapidil was used for α1A-adrenergic receptor blockade in dosages, which did not modify the MABP-response to blood loss. Western blot and quantitative reverse transcription polymerase chain reactions were used to determine adrenergic receptor expression in brain arterioles. Results: During hypovolemia subcortical CBF was maintained at 81 ± 6% of baseline, whereas cortical CBF decreased to 40 ± 4% (p < 0.001). Reperfusion led to peak CBFs of about 70% above baseline in both brain regions. α1A-Adrenergic blockade massively reduced subcortical CBF during hemorrhage and reperfusion, and prevented hyperperfusion during reperfusion in the cortex. α1A-mRNA expression was significantly higher in the cortex, whereas α1D-mRNA expression was higher in the subcortex (p < 0.001). Conclusions: α1-Adrenergic receptors are critical for perfusion redistribution: activity of the α1A-receptor subtype is a prerequisite for redistribution of CBF, whereas the α1D-receptor subtype may determine the magnitude of redistribution responses. |
topic |
adrenergic regulation alpha-adrenergic cerebral blood flow cerebral hemodynamics resuscitation head trauma cerebrovasvular disease neurodegenerative disease |
url |
http://www.mdpi.com/1422-0067/18/5/1031 |
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