Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting
Abstract Background Periodontitis is among the most common chronic diseases worldwide, and it is one of the main reasons for tooth loss. Comprehensive profiling of the metabolite content of the saliva can enable the identification of novel pathways associated with periodontitis and highlight non-inv...
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doaj-d5ce353b711846a7b1eed48545cbe6692021-07-18T11:19:02ZengBMCBMC Medicine1741-70152021-07-0119111310.1186/s12916-021-02035-zSalivary metabolites associated with a 5-year tooth loss identified in a population-based settingLeonie Andörfer0Birte Holtfreter1Stefan Weiss2Rutger Matthes3Vinay Pitchika4Carsten Oliver Schmidt5Stefanie Samietz6Gabi Kastenmüller7Matthias Nauck8Uwe Völker9Henry Völzke10Laszlo N. Csonka11Karsten Suhre12Maik Pietzner13Thomas Kocher14Department of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldInterfaculty Institute for Genetics and Functional Genomics, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldInstitute for Community Medicine, SHIP/Clinical Epidemiology Research, University Medicine GreifswaldDepartment of Prosthetic Dentistry, Gerodontology and Biomaterials, University Medicine GreifswaldInstitute of Bioinformatics and Systems Biology, Helmholtz Zentrum MünchenDZHK (German Center for Cardiovascular Research), partner site GreifswaldInterfaculty Institute for Genetics and Functional Genomics, University Medicine GreifswaldDZHK (German Center for Cardiovascular Research), partner site GreifswaldDepartment of Biological Sciences, Purdue UniversityInstitute of Bioinformatics and Systems Biology, Helmholtz Zentrum MünchenInstitute of Clinical Chemistry and Laboratory Medicine, University Medicine GreifswaldDepartment of Restorative Dentistry, Periodontology, Endodontology, and Preventive and Pediatric Dentistry, University Medicine GreifswaldAbstract Background Periodontitis is among the most common chronic diseases worldwide, and it is one of the main reasons for tooth loss. Comprehensive profiling of the metabolite content of the saliva can enable the identification of novel pathways associated with periodontitis and highlight non-invasive markers to facilitate time and cost-effective screening efforts for the presence of periodontitis and the prediction of tooth loss. Methods We first investigated cross-sectional associations of 13 oral health variables with saliva levels of 562 metabolites, measured by untargeted mass spectrometry among a sub-sample (n = 938) of the Study of Health in Pomerania (SHIP-2) using linear regression models adjusting for common confounders. We took forward any candidate metabolite associated with at least two oral variables, to test for an association with a 5-year tooth loss over and above baseline oral health status using negative binomial regression models. Results We identified 84 saliva metabolites that were associated with at least one oral variable cross-sectionally, for a subset of which we observed robust replication in an independent study. Out of 34 metabolites associated with more than two oral variables, baseline saliva levels of nine metabolites were positively associated with a 5-year tooth loss. Across all analyses, the metabolites 2-pyrrolidineacetic acid and butyrylputrescine were the most consistent candidate metabolites, likely reflecting oral dysbiosis. Other candidate metabolites likely reflected tissue destruction and cell proliferation. Conclusions Untargeted metabolic profiling of saliva replicated metabolic signatures of periodontal status and revealed novel metabolites associated with periodontitis and future tooth loss.https://doi.org/10.1186/s12916-021-02035-zMetabolomicsPeriodontitisProgressionTooth loss2-Pyrrolidineacetic acidButyrylputrescine |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leonie Andörfer Birte Holtfreter Stefan Weiss Rutger Matthes Vinay Pitchika Carsten Oliver Schmidt Stefanie Samietz Gabi Kastenmüller Matthias Nauck Uwe Völker Henry Völzke Laszlo N. Csonka Karsten Suhre Maik Pietzner Thomas Kocher |
spellingShingle |
Leonie Andörfer Birte Holtfreter Stefan Weiss Rutger Matthes Vinay Pitchika Carsten Oliver Schmidt Stefanie Samietz Gabi Kastenmüller Matthias Nauck Uwe Völker Henry Völzke Laszlo N. Csonka Karsten Suhre Maik Pietzner Thomas Kocher Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting BMC Medicine Metabolomics Periodontitis Progression Tooth loss 2-Pyrrolidineacetic acid Butyrylputrescine |
author_facet |
Leonie Andörfer Birte Holtfreter Stefan Weiss Rutger Matthes Vinay Pitchika Carsten Oliver Schmidt Stefanie Samietz Gabi Kastenmüller Matthias Nauck Uwe Völker Henry Völzke Laszlo N. Csonka Karsten Suhre Maik Pietzner Thomas Kocher |
author_sort |
Leonie Andörfer |
title |
Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
title_short |
Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
title_full |
Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
title_fullStr |
Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
title_full_unstemmed |
Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
title_sort |
salivary metabolites associated with a 5-year tooth loss identified in a population-based setting |
publisher |
BMC |
series |
BMC Medicine |
issn |
1741-7015 |
publishDate |
2021-07-01 |
description |
Abstract Background Periodontitis is among the most common chronic diseases worldwide, and it is one of the main reasons for tooth loss. Comprehensive profiling of the metabolite content of the saliva can enable the identification of novel pathways associated with periodontitis and highlight non-invasive markers to facilitate time and cost-effective screening efforts for the presence of periodontitis and the prediction of tooth loss. Methods We first investigated cross-sectional associations of 13 oral health variables with saliva levels of 562 metabolites, measured by untargeted mass spectrometry among a sub-sample (n = 938) of the Study of Health in Pomerania (SHIP-2) using linear regression models adjusting for common confounders. We took forward any candidate metabolite associated with at least two oral variables, to test for an association with a 5-year tooth loss over and above baseline oral health status using negative binomial regression models. Results We identified 84 saliva metabolites that were associated with at least one oral variable cross-sectionally, for a subset of which we observed robust replication in an independent study. Out of 34 metabolites associated with more than two oral variables, baseline saliva levels of nine metabolites were positively associated with a 5-year tooth loss. Across all analyses, the metabolites 2-pyrrolidineacetic acid and butyrylputrescine were the most consistent candidate metabolites, likely reflecting oral dysbiosis. Other candidate metabolites likely reflected tissue destruction and cell proliferation. Conclusions Untargeted metabolic profiling of saliva replicated metabolic signatures of periodontal status and revealed novel metabolites associated with periodontitis and future tooth loss. |
topic |
Metabolomics Periodontitis Progression Tooth loss 2-Pyrrolidineacetic acid Butyrylputrescine |
url |
https://doi.org/10.1186/s12916-021-02035-z |
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