Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans
Toxoplasmosis is a widely distributed zoonotic infection caused by the obligate intracellular apicomplexan parasite Toxoplasma gondii. It is mainly transmitted through the ingestion of oocysts shed by an infected cat acting as its definitive host. The key to effective control and treatment of toxopl...
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doaj-d5da6647f820498aac526bb003f7a9d82020-11-25T03:10:03ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-05-011010.3389/fcimb.2020.00204529812Review on the Current Trends of Toxoplasmosis Serodiagnosis in HumansRochelle Haidee D. Ybañez0Adrian P. Ybañez1Yoshifumi Nishikawa2National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanInstitute of Molecular Parasitology and Protozoan Diseases at Main and College of Veterinary Medicine, Cebu Technological University, Cebu City, PhilippinesNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, JapanToxoplasmosis is a widely distributed zoonotic infection caused by the obligate intracellular apicomplexan parasite Toxoplasma gondii. It is mainly transmitted through the ingestion of oocysts shed by an infected cat acting as its definitive host. The key to effective control and treatment of toxoplasmosis is prompt and accurate detection of T. gondii infection. Several laboratory diagnostic methods have been established, including the most commonly used serological assays such as the dye test (DT), direct or modified agglutination test (DAT/MAT), indirect hemagglutination test (IHA), latex agglutination test (LAT), indirect immunofluorescent test (IFAT), enzyme-linked immunosorbent assays (ELISA), immunochromatographic tests (ICT), and the western blot. Nonetheless, creating specific and reliable approaches for serodiagnosis of T. gondii infection, and differentiating between acute and chronic phases of infection remains a challenge. This review provides information on the current trends in the serodiagnosis of human toxoplasmosis. It highlights the advantages of the use of recombinant proteins for serological testing and provides insight into the possible future direction of these methods.https://www.frontiersin.org/article/10.3389/fcimb.2020.00204/fullToxoplasma gondiitoxoplasmosisserodiagnosisrecombinant antigenshuman |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rochelle Haidee D. Ybañez Adrian P. Ybañez Yoshifumi Nishikawa |
spellingShingle |
Rochelle Haidee D. Ybañez Adrian P. Ybañez Yoshifumi Nishikawa Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans Frontiers in Cellular and Infection Microbiology Toxoplasma gondii toxoplasmosis serodiagnosis recombinant antigens human |
author_facet |
Rochelle Haidee D. Ybañez Adrian P. Ybañez Yoshifumi Nishikawa |
author_sort |
Rochelle Haidee D. Ybañez |
title |
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans |
title_short |
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans |
title_full |
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans |
title_fullStr |
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans |
title_full_unstemmed |
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans |
title_sort |
review on the current trends of toxoplasmosis serodiagnosis in humans |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2020-05-01 |
description |
Toxoplasmosis is a widely distributed zoonotic infection caused by the obligate intracellular apicomplexan parasite Toxoplasma gondii. It is mainly transmitted through the ingestion of oocysts shed by an infected cat acting as its definitive host. The key to effective control and treatment of toxoplasmosis is prompt and accurate detection of T. gondii infection. Several laboratory diagnostic methods have been established, including the most commonly used serological assays such as the dye test (DT), direct or modified agglutination test (DAT/MAT), indirect hemagglutination test (IHA), latex agglutination test (LAT), indirect immunofluorescent test (IFAT), enzyme-linked immunosorbent assays (ELISA), immunochromatographic tests (ICT), and the western blot. Nonetheless, creating specific and reliable approaches for serodiagnosis of T. gondii infection, and differentiating between acute and chronic phases of infection remains a challenge. This review provides information on the current trends in the serodiagnosis of human toxoplasmosis. It highlights the advantages of the use of recombinant proteins for serological testing and provides insight into the possible future direction of these methods. |
topic |
Toxoplasma gondii toxoplasmosis serodiagnosis recombinant antigens human |
url |
https://www.frontiersin.org/article/10.3389/fcimb.2020.00204/full |
work_keys_str_mv |
AT rochellehaideedybanez reviewonthecurrenttrendsoftoxoplasmosisserodiagnosisinhumans AT adrianpybanez reviewonthecurrenttrendsoftoxoplasmosisserodiagnosisinhumans AT yoshifuminishikawa reviewonthecurrenttrendsoftoxoplasmosisserodiagnosisinhumans |
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