Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells
Colon cancer is one of the causes of cancer-related mortality. So, more efficient therapy strategies are needed. There is an increasing interest in natural products due to their potential cytotoxic activity in various cancer cell lines. Osthole and imperatorin are major active coumarins found in a v...
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doaj-d60098429c4f400dab226949702cbd842020-11-25T00:07:27ZengMDPI AGProceedings2504-39002017-11-01110100310.3390/proceedings1101003proceedings1101003Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma CellsAyşe Eken0Ayşe Kübra Karaboğa Arslan1Ebru Öztürk2Aysun Ökçesiz3Mükerrem Betül Yerer4Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, TurkeyDepartment of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, TurkeyDepartment of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, TurkeyDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, TurkeyDepartment of Pharmacology, Faculty of Pharmacy, Erciyes University, Kayseri 38039, TurkeyColon cancer is one of the causes of cancer-related mortality. So, more efficient therapy strategies are needed. There is an increasing interest in natural products due to their potential cytotoxic activity in various cancer cell lines. Osthole and imperatorin are major active coumarins found in a variety of plants. The aim of the present study was to assess the cytotoxic effects of osthole and imperatorin administered separately and in combination with 5-fluorouracil (5-FU) in human colon carcinoma cells and to identify the action of mechanism. Therefore, Colo205 cells were treated with imperatorin (200 μM), osthole (400 μM), and 5-FU (64 μM) 24 h after cell seeding. Real-time cell analysis by xCELLigence System was used to continuously monitor the cell proliferation and viability on Colo205 cells. Furthermore, the effects of the compound on p38 MAPK acitivity and Akt mRNA levels were evaluated. As a result, osthole showed considerable anti-proliferative activity in Colo205 cells and increased the efficacy of 5-FU by accelerating the cytotoxic effect and decreasing the Akt mRNA levels and inhibiting the p38 MAPK activity. Additionally, it was observed that osthole was more effective than imperatorin inhibiting cell proliferation. The findings indicate that osthole may be a promising anti-cancer agent in the treatment of colon cancer.https://www.mdpi.com/2504-3900/1/10/1003imperatorinosthole5-fluorouracilcytotoxicityxCELLigence |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ayşe Eken Ayşe Kübra Karaboğa Arslan Ebru Öztürk Aysun Ökçesiz Mükerrem Betül Yerer |
spellingShingle |
Ayşe Eken Ayşe Kübra Karaboğa Arslan Ebru Öztürk Aysun Ökçesiz Mükerrem Betül Yerer Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells Proceedings imperatorin osthole 5-fluorouracil cytotoxicity xCELLigence |
author_facet |
Ayşe Eken Ayşe Kübra Karaboğa Arslan Ebru Öztürk Aysun Ökçesiz Mükerrem Betül Yerer |
author_sort |
Ayşe Eken |
title |
Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells |
title_short |
Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells |
title_full |
Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells |
title_fullStr |
Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells |
title_full_unstemmed |
Cytotoxic Effects of Coumarin Compounds Imperatorin and Osthole, Alone and in Combination with 5-Fluorouracil in Colon Carcinoma Cells |
title_sort |
cytotoxic effects of coumarin compounds imperatorin and osthole, alone and in combination with 5-fluorouracil in colon carcinoma cells |
publisher |
MDPI AG |
series |
Proceedings |
issn |
2504-3900 |
publishDate |
2017-11-01 |
description |
Colon cancer is one of the causes of cancer-related mortality. So, more efficient therapy strategies are needed. There is an increasing interest in natural products due to their potential cytotoxic activity in various cancer cell lines. Osthole and imperatorin are major active coumarins found in a variety of plants. The aim of the present study was to assess the cytotoxic effects of osthole and imperatorin administered separately and in combination with 5-fluorouracil (5-FU) in human colon carcinoma cells and to identify the action of mechanism. Therefore, Colo205 cells were treated with imperatorin (200 μM), osthole (400 μM), and 5-FU (64 μM) 24 h after cell seeding. Real-time cell analysis by xCELLigence System was used to continuously monitor the cell proliferation and viability on Colo205 cells. Furthermore, the effects of the compound on p38 MAPK acitivity and Akt mRNA levels were evaluated. As a result, osthole showed considerable anti-proliferative activity in Colo205 cells and increased the efficacy of 5-FU by accelerating the cytotoxic effect and decreasing the Akt mRNA levels and inhibiting the p38 MAPK activity. Additionally, it was observed that osthole was more effective than imperatorin inhibiting cell proliferation. The findings indicate that osthole may be a promising anti-cancer agent in the treatment of colon cancer. |
topic |
imperatorin osthole 5-fluorouracil cytotoxicity xCELLigence |
url |
https://www.mdpi.com/2504-3900/1/10/1003 |
work_keys_str_mv |
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