Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification.
Heterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO includi...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4975503?pdf=render |
id |
doaj-d6079ec9529d46d78acdb2c93306ced9 |
---|---|
record_format |
Article |
spelling |
doaj-d6079ec9529d46d78acdb2c93306ced92020-11-25T00:08:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01118e015625310.1371/journal.pone.0156253Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification.Shailesh AgarwalJames DrakeAmmar T QureshiShawn LoderShuli LiKay ShigemoriJonathan PetersonDavid CholokJonathan A ForsbergYuji MishinaThomas A DavisBenjamin LeviHeterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO including two models of trauma-induced HO and one model of genetic HO, and human HO specimens, to characterize the properties of cells derived from tissue containing pre-and mature ectopic bone in relation to analogous mesenchymal cell populations or osteoblasts obtained from normal muscle tissue. We found that when cultured in vitro, cells isolated from the trauma sites in two distinct models exhibited increased osteogenic differentiation when compared to cells isolated from uninjured controls. Furthermore, osteoblasts isolated from heterotopic bone in a genetic model of HO also exhibited increased osteogenic differentiation when compared with normal osteoblasts. Finally, osteoblasts derived from mature heterotopic bone obtained from human patients exhibited increased osteogenic differentiation when compared with normal bone from the same patients. These findings demonstrate that across models, cells derived from tissues forming heterotopic ossification exhibit increased osteogenic differentiation when compared with either normal tissues or osteoblasts. These cell types can be used in the future for in vitro investigations for drug screening purposes.http://europepmc.org/articles/PMC4975503?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shailesh Agarwal James Drake Ammar T Qureshi Shawn Loder Shuli Li Kay Shigemori Jonathan Peterson David Cholok Jonathan A Forsberg Yuji Mishina Thomas A Davis Benjamin Levi |
spellingShingle |
Shailesh Agarwal James Drake Ammar T Qureshi Shawn Loder Shuli Li Kay Shigemori Jonathan Peterson David Cholok Jonathan A Forsberg Yuji Mishina Thomas A Davis Benjamin Levi Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. PLoS ONE |
author_facet |
Shailesh Agarwal James Drake Ammar T Qureshi Shawn Loder Shuli Li Kay Shigemori Jonathan Peterson David Cholok Jonathan A Forsberg Yuji Mishina Thomas A Davis Benjamin Levi |
author_sort |
Shailesh Agarwal |
title |
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. |
title_short |
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. |
title_full |
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. |
title_fullStr |
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. |
title_full_unstemmed |
Characterization of Cells Isolated from Genetic and Trauma-Induced Heterotopic Ossification. |
title_sort |
characterization of cells isolated from genetic and trauma-induced heterotopic ossification. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2016-01-01 |
description |
Heterotopic ossification (HO) is the pathologic formation of bone separate from the normal skeleton. Although several models exist for studying HO, an understanding of the common in vitro properties of cells isolated from these models is lacking. We studied three separate animal models of HO including two models of trauma-induced HO and one model of genetic HO, and human HO specimens, to characterize the properties of cells derived from tissue containing pre-and mature ectopic bone in relation to analogous mesenchymal cell populations or osteoblasts obtained from normal muscle tissue. We found that when cultured in vitro, cells isolated from the trauma sites in two distinct models exhibited increased osteogenic differentiation when compared to cells isolated from uninjured controls. Furthermore, osteoblasts isolated from heterotopic bone in a genetic model of HO also exhibited increased osteogenic differentiation when compared with normal osteoblasts. Finally, osteoblasts derived from mature heterotopic bone obtained from human patients exhibited increased osteogenic differentiation when compared with normal bone from the same patients. These findings demonstrate that across models, cells derived from tissues forming heterotopic ossification exhibit increased osteogenic differentiation when compared with either normal tissues or osteoblasts. These cell types can be used in the future for in vitro investigations for drug screening purposes. |
url |
http://europepmc.org/articles/PMC4975503?pdf=render |
work_keys_str_mv |
AT shaileshagarwal characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT jamesdrake characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT ammartqureshi characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT shawnloder characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT shulili characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT kayshigemori characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT jonathanpeterson characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT davidcholok characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT jonathanaforsberg characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT yujimishina characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT thomasadavis characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification AT benjaminlevi characterizationofcellsisolatedfromgeneticandtraumainducedheterotopicossification |
_version_ |
1725415531028152320 |