FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)
ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at interc...
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Academia Brasileira de Ciências
2016-03-01
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doaj-d6106ddf70e947fe913b8e602c2acbd92020-11-24T23:09:45ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902016-03-0188111712510.1590/0001-37652301620140616S0001-37652016000100117FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae)PATRICIA M. AGUILERAHUMBERTO J. DEBATMARISEL A. SCALDAFERRODARDO A. MARTÍMAURO GRABIELEABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117&lng=en&tlng=enCapsicum cultivados e silvestresgene de rRNA 5Ssonda derivada do Capsicummapeamento FISH |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
PATRICIA M. AGUILERA HUMBERTO J. DEBAT MARISEL A. SCALDAFERRO DARDO A. MARTÍ MAURO GRABIELE |
spellingShingle |
PATRICIA M. AGUILERA HUMBERTO J. DEBAT MARISEL A. SCALDAFERRO DARDO A. MARTÍ MAURO GRABIELE FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) Anais da Academia Brasileira de Ciências Capsicum cultivados e silvestres gene de rRNA 5S sonda derivada do Capsicum mapeamento FISH |
author_facet |
PATRICIA M. AGUILERA HUMBERTO J. DEBAT MARISEL A. SCALDAFERRO DARDO A. MARTÍ MAURO GRABIELE |
author_sort |
PATRICIA M. AGUILERA |
title |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_short |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_full |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_fullStr |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_full_unstemmed |
FISH-mapping of the 5S rDNA locus in chili peppers (Capsicum-Solanaceae) |
title_sort |
fish-mapping of the 5s rdna locus in chili peppers (capsicum-solanaceae) |
publisher |
Academia Brasileira de Ciências |
series |
Anais da Academia Brasileira de Ciências |
issn |
1678-2690 |
publishDate |
2016-03-01 |
description |
ABSTRACT We present here the physical mapping of the 5S rDNA locus in six wild and five cultivated taxa of Capsicum by means of a genus-specific FISH probe. In all taxa, a single 5S locus per haploid genome that persistently mapped onto the short arm of a unique metacentric chromosome pair at intercalar position, was found. 5S FISH signals of almost the same size and brightness intensity were observed in all the analyzed taxa. This is the first cytological characterization of the 5S in wild taxa of Capsicum by using a genus-derived probe, and the most exhaustive and comprehensive in the chili peppers up to now. The information provided here will aid the cytomolecular characterization of pepper germplasm to evaluate variability and can be instrumental to integrate physical, genetic and genomic maps already generated in the genus. |
topic |
Capsicum cultivados e silvestres gene de rRNA 5S sonda derivada do Capsicum mapeamento FISH |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100117&lng=en&tlng=en |
work_keys_str_mv |
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