PRAP: Pan Resistome analysis pipeline
Abstract Background Antibiotic resistance genes (ARGs) can spread among pathogens via horizontal gene transfer, resulting in imparities in their distribution even within the same species. Therefore, a pan-genome approach to analyzing resistomes is necessary for thoroughly characterizing patterns of...
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doaj-d619ffe9e4e14cb8a756e8d1b97c5eb12021-01-17T12:59:26ZengBMCBMC Bioinformatics1471-21052020-01-012111810.1186/s12859-019-3335-yPRAP: Pan Resistome analysis pipelineYichen He0Xiujuan Zhou1Ziyan Chen2Xiangyu Deng3Andrew Gehring4Hongyu Ou5Lida Zhang6Xianming Shi7Department of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityDepartment of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityDepartment of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityCenter for Food Safety, Department of Food Science and Technology, University of GeorgiaUnited States Department of Agriculture, Agricultural Research Service, Eastern Regional Research CenterDepartment of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityDepartment of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityDepartment of Food Science and Technology, MOST-USDA Joint Research Center for Food Safety, School of Agriculture & Biology, and State Key Lab of Microbial Metabolism, Shanghai Jiao Tong UniversityAbstract Background Antibiotic resistance genes (ARGs) can spread among pathogens via horizontal gene transfer, resulting in imparities in their distribution even within the same species. Therefore, a pan-genome approach to analyzing resistomes is necessary for thoroughly characterizing patterns of ARGs distribution within particular pathogen populations. Software tools are readily available for either ARGs identification or pan-genome analysis, but few exist to combine the two functions. Results We developed Pan Resistome Analysis Pipeline (PRAP) for the rapid identification of antibiotic resistance genes from various formats of whole genome sequences based on the CARD or ResFinder databases. Detailed annotations were used to analyze pan-resistome features and characterize distributions of ARGs. The contribution of different alleles to antibiotic resistance was predicted by a random forest classifier. Results of analysis were presented in browsable files along with a variety of visualization options. We demonstrated the performance of PRAP by analyzing the genomes of 26 Salmonella enterica isolates from Shanghai, China. Conclusions PRAP was effective for identifying ARGs and visualizing pan-resistome features, therefore facilitating pan-genomic investigation of ARGs. This tool has the ability to further excavate potential relationships between antibiotic resistance genes and their phenotypic traits.https://doi.org/10.1186/s12859-019-3335-yPan-resistomeIdentificationVisualizationMachine learning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yichen He Xiujuan Zhou Ziyan Chen Xiangyu Deng Andrew Gehring Hongyu Ou Lida Zhang Xianming Shi |
spellingShingle |
Yichen He Xiujuan Zhou Ziyan Chen Xiangyu Deng Andrew Gehring Hongyu Ou Lida Zhang Xianming Shi PRAP: Pan Resistome analysis pipeline BMC Bioinformatics Pan-resistome Identification Visualization Machine learning |
author_facet |
Yichen He Xiujuan Zhou Ziyan Chen Xiangyu Deng Andrew Gehring Hongyu Ou Lida Zhang Xianming Shi |
author_sort |
Yichen He |
title |
PRAP: Pan Resistome analysis pipeline |
title_short |
PRAP: Pan Resistome analysis pipeline |
title_full |
PRAP: Pan Resistome analysis pipeline |
title_fullStr |
PRAP: Pan Resistome analysis pipeline |
title_full_unstemmed |
PRAP: Pan Resistome analysis pipeline |
title_sort |
prap: pan resistome analysis pipeline |
publisher |
BMC |
series |
BMC Bioinformatics |
issn |
1471-2105 |
publishDate |
2020-01-01 |
description |
Abstract Background Antibiotic resistance genes (ARGs) can spread among pathogens via horizontal gene transfer, resulting in imparities in their distribution even within the same species. Therefore, a pan-genome approach to analyzing resistomes is necessary for thoroughly characterizing patterns of ARGs distribution within particular pathogen populations. Software tools are readily available for either ARGs identification or pan-genome analysis, but few exist to combine the two functions. Results We developed Pan Resistome Analysis Pipeline (PRAP) for the rapid identification of antibiotic resistance genes from various formats of whole genome sequences based on the CARD or ResFinder databases. Detailed annotations were used to analyze pan-resistome features and characterize distributions of ARGs. The contribution of different alleles to antibiotic resistance was predicted by a random forest classifier. Results of analysis were presented in browsable files along with a variety of visualization options. We demonstrated the performance of PRAP by analyzing the genomes of 26 Salmonella enterica isolates from Shanghai, China. Conclusions PRAP was effective for identifying ARGs and visualizing pan-resistome features, therefore facilitating pan-genomic investigation of ARGs. This tool has the ability to further excavate potential relationships between antibiotic resistance genes and their phenotypic traits. |
topic |
Pan-resistome Identification Visualization Machine learning |
url |
https://doi.org/10.1186/s12859-019-3335-y |
work_keys_str_mv |
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