Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.

Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.

Mesenchymal stem/stromal cells respond to physical cues present in their microenvironment such as substrate elasticity, geometry, or topography with respect to morphology, proliferation, and differentiation. Although studies have demonstrated the role of focal adhesions in topography-mediated change...

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Main Authors: Karina Kulangara, Jennifer Yang, Malathi Chellappan, Yong Yang, Kam W Leong
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4270691?pdf=render
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spelling doaj-d62175a867bf481f9dff6848d22c72212020-11-25T01:52:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11469810.1371/journal.pone.0114698Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.Karina KulangaraJennifer YangMalathi ChellappanYong YangKam W LeongMesenchymal stem/stromal cells respond to physical cues present in their microenvironment such as substrate elasticity, geometry, or topography with respect to morphology, proliferation, and differentiation. Although studies have demonstrated the role of focal adhesions in topography-mediated changes of gene expression, information linking substrate topography to the nucleus remains scarce. Here we show by two-dimensional gel electrophoresis and western blotting that A-type lamins and retinoblastoma protein are downregulated in mesenchymal stem/stromal cells cultured on 350 nm gratings compared to planar substrates; these changes lead to a decrease in proliferation and changes in differentiation potential.http://europepmc.org/articles/PMC4270691?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Karina Kulangara
Jennifer Yang
Malathi Chellappan
Yong Yang
Kam W Leong
spellingShingle Karina Kulangara
Jennifer Yang
Malathi Chellappan
Yong Yang
Kam W Leong
Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
PLoS ONE
author_facet Karina Kulangara
Jennifer Yang
Malathi Chellappan
Yong Yang
Kam W Leong
author_sort Karina Kulangara
title Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
title_short Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
title_full Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
title_fullStr Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
title_full_unstemmed Nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
title_sort nanotopography alters nuclear protein expression, proliferation and differentiation of human mesenchymal stem/stromal cells.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2014-01-01
description Mesenchymal stem/stromal cells respond to physical cues present in their microenvironment such as substrate elasticity, geometry, or topography with respect to morphology, proliferation, and differentiation. Although studies have demonstrated the role of focal adhesions in topography-mediated changes of gene expression, information linking substrate topography to the nucleus remains scarce. Here we show by two-dimensional gel electrophoresis and western blotting that A-type lamins and retinoblastoma protein are downregulated in mesenchymal stem/stromal cells cultured on 350 nm gratings compared to planar substrates; these changes lead to a decrease in proliferation and changes in differentiation potential.
url http://europepmc.org/articles/PMC4270691?pdf=render
work_keys_str_mv AT karinakulangara nanotopographyaltersnuclearproteinexpressionproliferationanddifferentiationofhumanmesenchymalstemstromalcells
AT jenniferyang nanotopographyaltersnuclearproteinexpressionproliferationanddifferentiationofhumanmesenchymalstemstromalcells
AT malathichellappan nanotopographyaltersnuclearproteinexpressionproliferationanddifferentiationofhumanmesenchymalstemstromalcells
AT yongyang nanotopographyaltersnuclearproteinexpressionproliferationanddifferentiationofhumanmesenchymalstemstromalcells
AT kamwleong nanotopographyaltersnuclearproteinexpressionproliferationanddifferentiationofhumanmesenchymalstemstromalcells
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