Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates
Objectives: The aim of this study was to validate the interpretation of red blood cell indices in complete blood count (CBC) and high performance liquid chromatography (HPLC) results on cord blood samples in consecutive Omani neonates. Methods: Cord blood samples from 7,837 neonates, were analysed w...
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doaj-d6374ecf546243b3bb8b2096686bf6de2020-11-25T02:55:06ZengSultan Qaboos UniversitySultan Qaboos University Medical Journal 2075-051X2075-05282011-11-011144624691550Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonatesSalam Alkindi0Anil Pathare1Ali Al-Madhani2Shoaib Al-Zadjali3Hamood Al-Haddabi4Qamariya Al- Abri5David Gravell6Mariam Mathew7Rajagopal Krishnamoorthy8Department of Haematology, College of Medicine & Health Sciences, Sultan Qaboos University, Muscat, OmanDepartment of Haematology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Medicine, Sohar Hospital, Sohar, OmanDepartment of Haematology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Haematology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Haematology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Haematology, Sultan Qaboos University Hospital, Muscat, OmanDepartment of Obstetrics & Gynecology, Sultan Qaboos University Hospital, Muscat, OmanHôpital Robert Debré, INSERM U763, Paris, France.Objectives: The aim of this study was to validate the interpretation of red blood cell indices in complete blood count (CBC) and high performance liquid chromatography (HPLC) results on cord blood samples in consecutive Omani neonates. Methods: Cord blood samples from 7,837 neonates, were analysed with CBC and HPLC using the β-thalassaemia short programme. Direct sequencing of abnormal samples with HbS, HbD, HbE and HbC was performed to validate the HPLC results. Additionally, in cases with HbA <10%, the β-globin gene was directly sequenced for β-thalassaemia mutation analysis. Results: Overall, 4,042 subjects (51.58%) had normal HPLC (HbA 22.88+_8.03; HbF 77.02+_8.04), whereas the presence of Hb Barts in the remaining 3,795 cases (48.42%) indicated the presence of α-thalassaemia. No case of HbH was detected. In the former subgroup respectively, the mean Hb (15.38+_2.04 g/dl) red blood cell (RBC) count (4.69+_0.68 x 1012/l), Hct (50.5+_7.18%), mean corpuscular volume (MCV) (107.66+_7.75 fl), mean corpuscular haemoglobin (MCH) (33.31+_4.07 pg), mean corpuscular haemoglobin concentration (MCHC) (30.98+_3.44 g/dl), red cell distribution width (RDW) (17.01+_2.17%) whereas, in the latter group with α-thalassaemia, it was (14.79+_2.90 g/dl); (5.09+_0.77 x 1012/l); (49.7+_7.40%); (97.29+_13.8 fl); (29.74+_11.80 pg); (30.39+_3.6 g/dl), and (18.09+_2.56%) respectively. DNA sequencing of samples with abnormal haemoglobin could validate the CBC and HLPC interpretations in all cases. Conclusion: This is the first study comparing the hemoglobin and red cell indices in the cord blood from newborn Omani subjects with those from other countries in the region, showing comparable results to those seen in Saudi neonates. The study also validates the CBC and HPLC interpretations of the cord blood red cell indices in the Omani neonate. The incidence of α-thalassaemia diagnosed by the presence of Hb Barts in cord blood of neonates was 48.42%.https://journals.squ.edu.om/index.php/squmj/article/view/1625neonatalscreeningreference range, haemoglobinvariantsalpha-thalassaemia. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Salam Alkindi Anil Pathare Ali Al-Madhani Shoaib Al-Zadjali Hamood Al-Haddabi Qamariya Al- Abri David Gravell Mariam Mathew Rajagopal Krishnamoorthy |
spellingShingle |
Salam Alkindi Anil Pathare Ali Al-Madhani Shoaib Al-Zadjali Hamood Al-Haddabi Qamariya Al- Abri David Gravell Mariam Mathew Rajagopal Krishnamoorthy Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates Sultan Qaboos University Medical Journal neonatal screening reference range, haemoglobin variants alpha-thalassaemia. |
author_facet |
Salam Alkindi Anil Pathare Ali Al-Madhani Shoaib Al-Zadjali Hamood Al-Haddabi Qamariya Al- Abri David Gravell Mariam Mathew Rajagopal Krishnamoorthy |
author_sort |
Salam Alkindi |
title |
Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates |
title_short |
Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates |
title_full |
Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates |
title_fullStr |
Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates |
title_full_unstemmed |
Neonatal Screening : Mean haemoglobin and red cell indices in cord blood from Omani neonates |
title_sort |
neonatal screening : mean haemoglobin and red cell indices in cord blood from omani neonates |
publisher |
Sultan Qaboos University |
series |
Sultan Qaboos University Medical Journal |
issn |
2075-051X 2075-0528 |
publishDate |
2011-11-01 |
description |
Objectives: The aim of this study was to validate the interpretation of red blood cell indices in complete blood count (CBC) and high performance liquid chromatography (HPLC) results on cord blood samples in consecutive Omani neonates. Methods: Cord blood samples from 7,837 neonates, were analysed with CBC and HPLC using the β-thalassaemia short programme. Direct sequencing of abnormal samples with HbS, HbD, HbE and HbC was performed to validate the HPLC results. Additionally, in cases with HbA <10%, the β-globin gene was directly sequenced for β-thalassaemia mutation analysis. Results: Overall, 4,042 subjects (51.58%) had normal HPLC (HbA 22.88+_8.03; HbF 77.02+_8.04), whereas the presence of Hb Barts in the remaining 3,795 cases (48.42%) indicated the presence of α-thalassaemia. No case of HbH was detected. In the former subgroup respectively, the mean Hb (15.38+_2.04 g/dl) red blood cell (RBC) count (4.69+_0.68 x 1012/l), Hct (50.5+_7.18%), mean corpuscular volume (MCV) (107.66+_7.75 fl), mean corpuscular haemoglobin (MCH) (33.31+_4.07 pg), mean corpuscular haemoglobin concentration (MCHC) (30.98+_3.44 g/dl), red cell distribution width (RDW) (17.01+_2.17%) whereas, in the latter group with α-thalassaemia, it was (14.79+_2.90 g/dl); (5.09+_0.77 x 1012/l); (49.7+_7.40%); (97.29+_13.8 fl); (29.74+_11.80 pg); (30.39+_3.6 g/dl), and (18.09+_2.56%) respectively. DNA sequencing of samples with abnormal haemoglobin could validate the CBC and HLPC interpretations in all cases. Conclusion: This is the first study comparing the hemoglobin and red cell indices in the cord blood from newborn Omani subjects with those from other countries in the region, showing comparable results to those seen in Saudi neonates. The study also validates the CBC and HPLC interpretations of the cord blood red cell indices in the Omani neonate. The incidence of α-thalassaemia diagnosed by the presence of Hb Barts in cord blood of neonates was 48.42%. |
topic |
neonatal screening reference range, haemoglobin variants alpha-thalassaemia. |
url |
https://journals.squ.edu.om/index.php/squmj/article/view/1625 |
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