A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are...
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Format: | Article |
Language: | English |
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Hindawi Limited
2016-01-01
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Series: | Stem Cells International |
Online Access: | http://dx.doi.org/10.1155/2016/9042019 |
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doaj-d63d2fb0ff864f9b94892058f04e9716 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yibo Gan Sukai Li Pei Li Yuan Xu Liyuan Wang Chen Zhao Bin Ouyang Bing Tu Chengmin Zhang Lei Luo Xiangdong Luo Xiumei Mo Qiang Zhou |
spellingShingle |
Yibo Gan Sukai Li Pei Li Yuan Xu Liyuan Wang Chen Zhao Bin Ouyang Bing Tu Chengmin Zhang Lei Luo Xiangdong Luo Xiumei Mo Qiang Zhou A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration Stem Cells International |
author_facet |
Yibo Gan Sukai Li Pei Li Yuan Xu Liyuan Wang Chen Zhao Bin Ouyang Bing Tu Chengmin Zhang Lei Luo Xiangdong Luo Xiumei Mo Qiang Zhou |
author_sort |
Yibo Gan |
title |
A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration |
title_short |
A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration |
title_full |
A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration |
title_fullStr |
A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration |
title_full_unstemmed |
A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus Regeneration |
title_sort |
controlled release codelivery system of mscs encapsulated in dextran/gelatin hydrogel with tgf-β3-loaded nanoparticles for nucleus pulposus regeneration |
publisher |
Hindawi Limited |
series |
Stem Cells International |
issn |
1687-966X 1687-9678 |
publishDate |
2016-01-01 |
description |
Mesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP. The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation. TGF-β3-loaded PLGA nanoparticles were prepared by the double-emulsion solvent evaporation method and seeded uniformly into the hydrogel. Morphological observations, an assessment of the release kinetics of TGF-β3, a cytotoxic assay, a cell proliferation test, a biochemical content assay, qRT-PCR, and immunohistological analyses of the codelivery system were conducted in the study. The results showed that the TGF-β3-loaded nanoparticles could release TGF-β3 gradually. The codelivery system exhibited favorable cytocompatibility, and the TGF-β3 that was released could induce MSCs to NP-like cells while promoting ECM-related biosynthesis. These results suggest this codelivery system may be employed as a promising carrier for discogenesis of MSCs in situ. |
url |
http://dx.doi.org/10.1155/2016/9042019 |
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doaj-d63d2fb0ff864f9b94892058f04e97162020-11-24T23:48:01ZengHindawi LimitedStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/90420199042019A Controlled Release Codelivery System of MSCs Encapsulated in Dextran/Gelatin Hydrogel with TGF-β3-Loaded Nanoparticles for Nucleus Pulposus RegenerationYibo Gan0Sukai Li1Pei Li2Yuan Xu3Liyuan Wang4Chen Zhao5Bin Ouyang6Bing Tu7Chengmin Zhang8Lei Luo9Xiangdong Luo10Xiumei Mo11Qiang Zhou12National & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaDepartment of Orthopaedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaInstitution of Burn Research, Southwest Hospital, Third Military Medical University, Chongqing 400038, ChinaCollege of Chemistry and Chemical Engineering and Biological Engineering, Donghua University, Shanghai 201620, ChinaNational & Regional United Engineering Laboratory of Tissue Engineering, Department of Orthopedics, Southwest Hospital, Third Military Medical University, 30 No. Gao Tan Yan Street, Shapingba District, Chongqing 400038, ChinaMesenchymal stem cell- (MSC-) based therapy is regarded as a potential tissue engineering strategy to achieve nucleus pulposus (NP) regeneration for the treatment of intervertebral disc degeneration (IDD). However, it is still a challenge to induce MSC differentiation in NP-like cells when MSCs are implanted into the NP. The purpose of this study was to construct poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles as carriers for TGF-β3 controlled release and establish a codelivery system of a dextran/gelatin hydrogel with the nanoparticles for long-term processing of discogenesis differentiation. TGF-β3-loaded PLGA nanoparticles were prepared by the double-emulsion solvent evaporation method and seeded uniformly into the hydrogel. Morphological observations, an assessment of the release kinetics of TGF-β3, a cytotoxic assay, a cell proliferation test, a biochemical content assay, qRT-PCR, and immunohistological analyses of the codelivery system were conducted in the study. The results showed that the TGF-β3-loaded nanoparticles could release TGF-β3 gradually. The codelivery system exhibited favorable cytocompatibility, and the TGF-β3 that was released could induce MSCs to NP-like cells while promoting ECM-related biosynthesis. These results suggest this codelivery system may be employed as a promising carrier for discogenesis of MSCs in situ.http://dx.doi.org/10.1155/2016/9042019 |