Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?

• Main Authors: Lisa Vork, John Penders, Jonna Jalanka, Svetlana Bojic, Sander M. J. van Kuijk, Anne Salonen, Willem M. de Vos, Mirjana Rajilic-Stojanovic, Zsa Zsa R. M. Weerts, Ad A. M. Masclee, Marta Pozuelo, Chaysavanh Manichanh, Daisy M. A. E. Jonkers
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2021-07-01
• Series: Frontiers in Cellular and Infection Microbiology
• Subjects: fecal microbiota; intestinal microbiome; stool consistency; irritable bowel syndrome; adult
• Online Access: https://www.frontiersin.org/articles/10.3389/fcimb.2021.639667/full

IntroductionStool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days.MethodsTwelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined.ResultsLinear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this.ConclusionThis study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual’s microbiota at a given time point. NCT00775060.