Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?
IntroductionStool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We...
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Frontiers Media S.A.
2021-07-01
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Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2021.639667/full |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lisa Vork John Penders Jonna Jalanka Svetlana Bojic Sander M. J. van Kuijk Anne Salonen Willem M. de Vos Willem M. de Vos Mirjana Rajilic-Stojanovic Zsa Zsa R. M. Weerts Ad A. M. Masclee Marta Pozuelo Chaysavanh Manichanh Chaysavanh Manichanh Daisy M. A. E. Jonkers |
spellingShingle |
Lisa Vork John Penders Jonna Jalanka Svetlana Bojic Sander M. J. van Kuijk Anne Salonen Willem M. de Vos Willem M. de Vos Mirjana Rajilic-Stojanovic Zsa Zsa R. M. Weerts Ad A. M. Masclee Marta Pozuelo Chaysavanh Manichanh Chaysavanh Manichanh Daisy M. A. E. Jonkers Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? Frontiers in Cellular and Infection Microbiology fecal microbiota intestinal microbiome stool consistency irritable bowel syndrome adult |
author_facet |
Lisa Vork John Penders Jonna Jalanka Svetlana Bojic Sander M. J. van Kuijk Anne Salonen Willem M. de Vos Willem M. de Vos Mirjana Rajilic-Stojanovic Zsa Zsa R. M. Weerts Ad A. M. Masclee Marta Pozuelo Chaysavanh Manichanh Chaysavanh Manichanh Daisy M. A. E. Jonkers |
author_sort |
Lisa Vork |
title |
Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? |
title_short |
Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? |
title_full |
Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? |
title_fullStr |
Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? |
title_full_unstemmed |
Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition? |
title_sort |
does day-to-day variability in stool consistency link to the fecal microbiota composition? |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cellular and Infection Microbiology |
issn |
2235-2988 |
publishDate |
2021-07-01 |
description |
IntroductionStool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days.MethodsTwelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined.ResultsLinear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this.ConclusionThis study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual’s microbiota at a given time point. NCT00775060. |
topic |
fecal microbiota intestinal microbiome stool consistency irritable bowel syndrome adult |
url |
https://www.frontiersin.org/articles/10.3389/fcimb.2021.639667/full |
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doaj-d64238ef5ca345e3834807415e212e5a2021-08-11T09:14:49ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882021-07-011110.3389/fcimb.2021.639667639667Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?Lisa Vork0John Penders1Jonna Jalanka2Svetlana Bojic3Sander M. J. van Kuijk4Anne Salonen5Willem M. de Vos6Willem M. de Vos7Mirjana Rajilic-Stojanovic8Zsa Zsa R. M. Weerts9Ad A. M. Masclee10Marta Pozuelo11Chaysavanh Manichanh12Chaysavanh Manichanh13Daisy M. A. E. Jonkers14Division of Gastroenterology-Hepatology, Department of Internal Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, NetherlandsDepartment of Medical Microbiology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, NetherlandsHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, FinlandDepartment of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, SerbiaClinical Epidemiology and Medical Technology Assessment, Maastricht University Medical Center+, Maastricht, NetherlandsHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, FinlandHuman Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, FinlandLaboratory of Microbiology, Wageningen University, Wageningen, NetherlandsDepartment of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, SerbiaDivision of Gastroenterology-Hepatology, Department of Internal Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, NetherlandsDivision of Gastroenterology-Hepatology, Department of Internal Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, NetherlandsDigestive System Research Unit, University Hospital Vall d’Hebron, Barcelona, SpainDigestive System Research Unit, University Hospital Vall d’Hebron, Barcelona, SpainCentro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, Madrid, SpainDivision of Gastroenterology-Hepatology, Department of Internal Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, NetherlandsIntroductionStool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days.MethodsTwelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined.ResultsLinear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this.ConclusionThis study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual’s microbiota at a given time point. NCT00775060.https://www.frontiersin.org/articles/10.3389/fcimb.2021.639667/fullfecal microbiotaintestinal microbiomestool consistencyirritable bowel syndromeadult |