Mini Gel Electrophoresis Control Software and System Design With Python

• Main Authors: Ahmet Reşit KAVSAOĞLU, İsmail MERSİNKAYA
• Format: Article
• Language: English
• Published: Gazi University 2019-12-01
• Series: Gazi Üniversitesi Fen Bilimleri Dergisi
• Subjects: python; control software; mini gel electrophoresis; embedded system
• Online Access: https://dergipark.org.tr/tr/download/article-file/878474

Electrophoresis is the method of separating charged macromolecules such as DNA, RNA and Protein molecules by moving them from one pole to the other, between (-) and (+) charged poles, in an electric field. In the classical electrophoresis process, devices and materials used for power supply, UV (ultraviolet) transilluminator and gel imaging, process control procedures are performed as separate processes and by the experimenters. Smile effect, diffusion effect and other factors may decrease the sensitivity in the analysis and may result in incorrect band image and band density calculation on the results. This study aims to increase the sensitivity of the analysis by minimizing the voltage fluctuation of the power supply during the electrophoresis process and the errors of the bands such as the bands overflowing out of the gel when the tester does not turn off the power supply in time; Control software, mini gel electrophoresis system design and application studies, which enable the devices used to operate in order of operation and the calculation operations can be performed automatically by a microprocessor and microcontroller interfaces. When the control software and classical electrophoresis processes were compared, positive results were obtained.