The Microbiome Associated with the Reef Builder <i>Neogoniolithon</i> sp. in the Eastern Mediterranean

• Main Authors: Shany Gefen-Treves, Alexander Bartholomäus, Fabian Horn, Adam Boleslaw Zaborowski, Dan Tchernov, Dirk Wagner, Aharon Oren, Aaron Kaplan
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Microorganisms
• Subjects: microbiome; reef builder; 16S rRNA sequencing; algal; bacteria; interaction
• Online Access: https://www.mdpi.com/2076-2607/9/7/1374

The development of coastal vermetid reefs and rocky shores depends on the activity of several reef builders, including red crustose coralline algae (CCA) such as <i>Neogoniolithon</i> sp. To initiate studies on the interaction between <i>Neogoniolithon</i> sp. and its associated bacteria, and their impact on the algae physiological performance, we characterized the bacterial community by 16S rRNA gene sequencing. These were extracted from the algal tissue and adjacent waters along two sampling campaigns (during winter and spring), in three study regions along a reef in the east Mediterranean Israeli coast and from laboratory-grown algae. The analysis revealed that aquaria and field communities differ substantially, suggesting that future research on <i>Neogoniolithon</i> sp. interaction with its microbiome must rest on aquaria that closely simulate coastal conditions. Some prokaryote classes found associated with the alga tissue were hardly detected or absent from surrounding water. Further, bacterial populations differed between sampling campaigns. One example is the presence of anaerobic bacteria and archaea families in one of the campaigns, correlating with the weaker turbulence in the spring season, probably leading to the development of local anoxic conditions. A better understanding of reef-building activity of CCA and their associated bacteria is necessary for assessment of their resilience to climate change and may support coastal preservation efforts.