New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35
Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2013-10-01
|
Series: | Marine Drugs |
Subjects: | |
Online Access: | http://www.mdpi.com/1660-3397/11/10/3891 |
id |
doaj-d6598fb665e24b12bf1bad10477249a7 |
---|---|
record_format |
Article |
spelling |
doaj-d6598fb665e24b12bf1bad10477249a72020-11-24T23:48:27ZengMDPI AGMarine Drugs1660-33972013-10-0111103891390110.3390/md11103891New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35Shu-Jin WangHui-Ming HuaJiang-Chun HuZhan-Lin LiNan WangSong-Ya ZhangHua-Qi PanStrain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin.http://www.mdpi.com/1660-3397/11/10/3891lobophorinsspirotetronate antibioticsantimicrobialStreptomycesSouth China Sea-derived actinomycete |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan |
spellingShingle |
Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 Marine Drugs lobophorins spirotetronate antibiotics antimicrobial Streptomyces South China Sea-derived actinomycete |
author_facet |
Shu-Jin Wang Hui-Ming Hua Jiang-Chun Hu Zhan-Lin Li Nan Wang Song-Ya Zhang Hua-Qi Pan |
author_sort |
Shu-Jin Wang |
title |
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
title_short |
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
title_full |
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
title_fullStr |
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
title_full_unstemmed |
New Spirotetronate Antibiotics, Lobophorins H and I, from a South China Sea-Derived Streptomyces sp. 12A35 |
title_sort |
new spirotetronate antibiotics, lobophorins h and i, from a south china sea-derived streptomyces sp. 12a35 |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2013-10-01 |
description |
Strain 12A35 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antibacterial activities. It was identified as Streptomyces sp. by the 16S rDNA sequence analysis. Bioassay-guided fractionation using HP20 adsorption, flash chromatography over silica gel and octadecylsilyl (ODS) and semi-preparative HPLC, led to the isolation and purification of five metabolites from the fermentation culture of 12A35. Two new spirotetronate antibiotics, lobophorins H (1) and I (2), along with three known analogues, O-β-kijanosyl-(1→17)-kijanolide (3), lobophorins B (4) and F (5) were characterized by 1D, 2D-NMR and MS data. These compounds exhibited significant inhibitory activities against Bacillus subtilis. Compounds 1 and 5 exhibited moderate activities against Staphylococcus aureus. In particular, the new compound lobophorin H (1) showed similar antibacterial activities against B. subtilis CMCC63501 to ampicillin. |
topic |
lobophorins spirotetronate antibiotics antimicrobial Streptomyces South China Sea-derived actinomycete |
url |
http://www.mdpi.com/1660-3397/11/10/3891 |
work_keys_str_mv |
AT shujinwang newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT huiminghua newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT jiangchunhu newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT zhanlinli newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT nanwang newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT songyazhang newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 AT huaqipan newspirotetronateantibioticslobophorinshandifromasouthchinaseaderivedstreptomycessp12a35 |
_version_ |
1725486077841506304 |