“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy
This contribution reports the design, preparation, photophysical and photochemical characterization, as well as a preliminary biological evaluation of mesoporous silica nanoparticles (MSNs) covalently integrating a nitric oxide (NO) photodonor (NOPD) and a singlet oxygen (<sup>1</sup>O&l...
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doaj-d66564fe98b54f8c9eafd3b32a880f1e2020-11-24T21:14:46ZengMDPI AGNanomaterials2079-49912019-05-019682310.3390/nano9060823nano9060823“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/ChemotherapyAndré Luiz Tessaro0Aurore Fraix1Ana Claudia Pedrozo da Silva2Elena Gazzano3Chiara Riganti4Salvatore Sortino5Laboratory of Photochemistry, Department of Drug Sciences, University of Catania, 95125 Catania, ItalyLaboratory of Photochemistry, Department of Drug Sciences, University of Catania, 95125 Catania, ItalyDepartment of Chemistry, Universidade Estadual de Maringá, Av. Colombo, 5.790, Maringá 87.020-900, Paraná, BrazilDepartment of Oncology, University of Torino, Via Santena 5/bis, I-10126 Torino, ItalyDepartment of Oncology, University of Torino, Via Santena 5/bis, I-10126 Torino, ItalyLaboratory of Photochemistry, Department of Drug Sciences, University of Catania, 95125 Catania, ItalyThis contribution reports the design, preparation, photophysical and photochemical characterization, as well as a preliminary biological evaluation of mesoporous silica nanoparticles (MSNs) covalently integrating a nitric oxide (NO) photodonor (NOPD) and a singlet oxygen (<sup>1</sup>O<sub>2</sub>) photosensitizer (PS) and encapsulating the anticancer doxorubicin (DOX) in a noncovalent fashion. These MSNs bind the NOPD mainly in their inner part and the PS in their outer part in order to judiciously exploit the different diffusion radius of the cytotoxic NO and <sup>1</sup>O<sub>2</sub>. Furthermore this silica nanoconstruct has been devised in such a way to permit the selective excitation of the NOPD and the PS with light sources of different energy in the visible window. We demonstrate that the individual photochemical performances of the photoactive components of the MSNs are not mutually affected, and remain unaltered even in the presence of DOX. As a result, the complete nanoconstruct is able to deliver NO and <sup>1</sup>O<sub>2</sub> under blue and green light, respectively, and to release DOX under physiological conditions. Preliminary biological results performed using A375 cancer cells show a good tolerability of the functionalized MSNs in the dark and a potentiated activity of DOX upon irradiation, due to the effect of the NO photoreleased.https://www.mdpi.com/2079-4991/9/6/823multimodal therapysinglet oxygendoxorubicinnitric oxide |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
André Luiz Tessaro Aurore Fraix Ana Claudia Pedrozo da Silva Elena Gazzano Chiara Riganti Salvatore Sortino |
spellingShingle |
André Luiz Tessaro Aurore Fraix Ana Claudia Pedrozo da Silva Elena Gazzano Chiara Riganti Salvatore Sortino “Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy Nanomaterials multimodal therapy singlet oxygen doxorubicin nitric oxide |
author_facet |
André Luiz Tessaro Aurore Fraix Ana Claudia Pedrozo da Silva Elena Gazzano Chiara Riganti Salvatore Sortino |
author_sort |
André Luiz Tessaro |
title |
“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy |
title_short |
“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy |
title_full |
“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy |
title_fullStr |
“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy |
title_full_unstemmed |
“Three-Bullets” Loaded Mesoporous Silica Nanoparticles for Combined Photo/Chemotherapy |
title_sort |
“three-bullets” loaded mesoporous silica nanoparticles for combined photo/chemotherapy |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2019-05-01 |
description |
This contribution reports the design, preparation, photophysical and photochemical characterization, as well as a preliminary biological evaluation of mesoporous silica nanoparticles (MSNs) covalently integrating a nitric oxide (NO) photodonor (NOPD) and a singlet oxygen (<sup>1</sup>O<sub>2</sub>) photosensitizer (PS) and encapsulating the anticancer doxorubicin (DOX) in a noncovalent fashion. These MSNs bind the NOPD mainly in their inner part and the PS in their outer part in order to judiciously exploit the different diffusion radius of the cytotoxic NO and <sup>1</sup>O<sub>2</sub>. Furthermore this silica nanoconstruct has been devised in such a way to permit the selective excitation of the NOPD and the PS with light sources of different energy in the visible window. We demonstrate that the individual photochemical performances of the photoactive components of the MSNs are not mutually affected, and remain unaltered even in the presence of DOX. As a result, the complete nanoconstruct is able to deliver NO and <sup>1</sup>O<sub>2</sub> under blue and green light, respectively, and to release DOX under physiological conditions. Preliminary biological results performed using A375 cancer cells show a good tolerability of the functionalized MSNs in the dark and a potentiated activity of DOX upon irradiation, due to the effect of the NO photoreleased. |
topic |
multimodal therapy singlet oxygen doxorubicin nitric oxide |
url |
https://www.mdpi.com/2079-4991/9/6/823 |
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