Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors
Biased integration remains a key challenge for gene therapy based on lentiviral vector technologies. Engineering of next-generation lentiviral vectors targeting safe genomic harbors for insertion is therefore of high relevance. In a previous paper (Cai et al., 2014a), we showed the use of integrase-...
Main Authors: | Yujia Cai, Anders Laustsen, Yan Zhou, Chenglong Sun, Mads Valdemar Anderson, Shengting Li, Niels Uldbjerg, Yonglun Luo, Martin R Jakobsen, Jacob Giehm Mikkelsen |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2016-06-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/12213 |
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