A rapid method for the generation of uniform acellular bone explants: a technical note
<p>Abstract</p> <p>Background</p> <p>Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants.</p> <p>Methods</p> <p>Therefore, ovine cancellous bon...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2010-05-01
|
Series: | Journal of Orthopaedic Surgery and Research |
Online Access: | http://www.josr-online.com/content/5/1/32 |
id |
doaj-d6954cf41b3b4a7f82590b2e3960cc75 |
---|---|
record_format |
Article |
spelling |
doaj-d6954cf41b3b4a7f82590b2e3960cc752020-11-24T23:27:18ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2010-05-01513210.1186/1749-799X-5-32A rapid method for the generation of uniform acellular bone explants: a technical noteSimpson Angharad EFurlong Pamela IBraunstein VolkerJähn KatharinaRichards R GeoffStoddart Martin J<p>Abstract</p> <p>Background</p> <p>Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants.</p> <p>Methods</p> <p>Therefore, ovine cancellous bone explants from the iliac crest were prepared and used to test several methods for the induction of cell death. Over night heat inactivation was used as positive treatment control, methods to be investigated included UV light, or X- ray exposure, incubation in a hypotonic solution (salt-free water) and a short cycle of repeated freezing and thawing.</p> <p>Results</p> <p>Viability of treated and 2 days cultured bone explants was investigated by lactate dehydrogenase assay. Non-treated cultured control explants maintained around 50% osteocyte viability, while osteocyte survival after the positive treatment control was abolished. The most dramatic loss in cell viability, together with a low standard deviation, was a repeated cycle of freezing and thawing.</p> <p>Conclusions</p> <p>To summarize, we present a freeze-thaw method for the creation of acellular bone explants, which is easy to perform, not time-consuming and provides consistent results.</p> http://www.josr-online.com/content/5/1/32 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Simpson Angharad E Furlong Pamela I Braunstein Volker Jähn Katharina Richards R Geoff Stoddart Martin J |
spellingShingle |
Simpson Angharad E Furlong Pamela I Braunstein Volker Jähn Katharina Richards R Geoff Stoddart Martin J A rapid method for the generation of uniform acellular bone explants: a technical note Journal of Orthopaedic Surgery and Research |
author_facet |
Simpson Angharad E Furlong Pamela I Braunstein Volker Jähn Katharina Richards R Geoff Stoddart Martin J |
author_sort |
Simpson Angharad E |
title |
A rapid method for the generation of uniform acellular bone explants: a technical note |
title_short |
A rapid method for the generation of uniform acellular bone explants: a technical note |
title_full |
A rapid method for the generation of uniform acellular bone explants: a technical note |
title_fullStr |
A rapid method for the generation of uniform acellular bone explants: a technical note |
title_full_unstemmed |
A rapid method for the generation of uniform acellular bone explants: a technical note |
title_sort |
rapid method for the generation of uniform acellular bone explants: a technical note |
publisher |
BMC |
series |
Journal of Orthopaedic Surgery and Research |
issn |
1749-799X |
publishDate |
2010-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Bone graft studies lack standardized controls. We aim to present a quick and reliable method for the intra-operative generation of acellular bone explants.</p> <p>Methods</p> <p>Therefore, ovine cancellous bone explants from the iliac crest were prepared and used to test several methods for the induction of cell death. Over night heat inactivation was used as positive treatment control, methods to be investigated included UV light, or X- ray exposure, incubation in a hypotonic solution (salt-free water) and a short cycle of repeated freezing and thawing.</p> <p>Results</p> <p>Viability of treated and 2 days cultured bone explants was investigated by lactate dehydrogenase assay. Non-treated cultured control explants maintained around 50% osteocyte viability, while osteocyte survival after the positive treatment control was abolished. The most dramatic loss in cell viability, together with a low standard deviation, was a repeated cycle of freezing and thawing.</p> <p>Conclusions</p> <p>To summarize, we present a freeze-thaw method for the creation of acellular bone explants, which is easy to perform, not time-consuming and provides consistent results.</p> |
url |
http://www.josr-online.com/content/5/1/32 |
work_keys_str_mv |
AT simpsonangharade arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT furlongpamelai arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT braunsteinvolker arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT jahnkatharina arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT richardsrgeoff arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT stoddartmartinj arapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT simpsonangharade rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT furlongpamelai rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT braunsteinvolker rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT jahnkatharina rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT richardsrgeoff rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote AT stoddartmartinj rapidmethodforthegenerationofuniformacellularboneexplantsatechnicalnote |
_version_ |
1725552451683090432 |