Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis
Osteoarthritis (OA) is a common chronic joint disease. This study aimed to explore the function of long noncoding RNA taurine-upregulated gene 1 (TUG1) in the progression and initiation of OA. Levels of TUG1, microRNA-320c (miR-320c) and fucosyltransferase 4 (FUT4) were examined via quantitative rev...
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Online Access: | https://doi.org/10.1515/biol-2021-0037 |
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doaj-d6957fb54a584f4c835ccd270c6cba902021-10-03T07:42:26ZengDe GruyterOpen Life Sciences2391-54122021-04-0116138439410.1515/biol-2021-0037Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritisHan Hu0Liu Lijuan1Department of Rehabilitation, The First People’s Hospital of Jingmen, No. 67 Xiangshan Dadao, Dongbao District, Jingmen 448000, Hubei, ChinaDepartment of Rehabilitation, The First People’s Hospital of Jingmen, No. 67 Xiangshan Dadao, Dongbao District, Jingmen 448000, Hubei, ChinaOsteoarthritis (OA) is a common chronic joint disease. This study aimed to explore the function of long noncoding RNA taurine-upregulated gene 1 (TUG1) in the progression and initiation of OA. Levels of TUG1, microRNA-320c (miR-320c) and fucosyltransferase 4 (FUT4) were examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry assays were used to detect cell viability and apoptosis, respectively. The expression of relative proteins was measured using Western blot. The interaction between miR-320c and TUG1 or FUT4 was confirmed utilizing dual-luciferase reporter and RNA immunoprecipitation assays. In this study, levels of TUG1 and FUT4 were distinctly upregulated, but miR-320c level significantly decreased in OA tissues and chondrocytes derived from OA tissues as well as in IL-1β-stimulated C28/I2 cells. Mechanically, TUG1 sponged miR-320c and miR-320c targeted FUT4. In addition, TUG1 knockdown accelerated cell proliferation and repressed apoptosis and extracellular matrix (ECM) degradation in IL-1β-induced C28/I2 cells, whereas these effects of TUG1 deletion were rescued by either miR-320c inhibitor or FUT4 upregulation. Meanwhile, TUG1 sponged miR-320c to regulate FUT4 expression in IL-1β-induced C28/I2 cells. Collectively, TUG1 modulated cell proliferation, apoptosis and ECM degradation in IL-1β-induced C28/I2 cells via the miR-320c/FUT4 axis, providing a new insight into the OA treatment.https://doi.org/10.1515/biol-2021-0037osteoarthritistug1mir-320cfut4il-1β |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Hu Liu Lijuan |
spellingShingle |
Han Hu Liu Lijuan Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis Open Life Sciences osteoarthritis tug1 mir-320c fut4 il-1β |
author_facet |
Han Hu Liu Lijuan |
author_sort |
Han Hu |
title |
Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis |
title_short |
Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis |
title_full |
Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis |
title_fullStr |
Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis |
title_full_unstemmed |
Long noncoding RNA TUG1 regulates degradation of chondrocyte extracellular matrix via miR-320c/MMP-13 axis in osteoarthritis |
title_sort |
long noncoding rna tug1 regulates degradation of chondrocyte extracellular matrix via mir-320c/mmp-13 axis in osteoarthritis |
publisher |
De Gruyter |
series |
Open Life Sciences |
issn |
2391-5412 |
publishDate |
2021-04-01 |
description |
Osteoarthritis (OA) is a common chronic joint disease. This study aimed to explore the function of long noncoding RNA taurine-upregulated gene 1 (TUG1) in the progression and initiation of OA. Levels of TUG1, microRNA-320c (miR-320c) and fucosyltransferase 4 (FUT4) were examined via quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry assays were used to detect cell viability and apoptosis, respectively. The expression of relative proteins was measured using Western blot. The interaction between miR-320c and TUG1 or FUT4 was confirmed utilizing dual-luciferase reporter and RNA immunoprecipitation assays. In this study, levels of TUG1 and FUT4 were distinctly upregulated, but miR-320c level significantly decreased in OA tissues and chondrocytes derived from OA tissues as well as in IL-1β-stimulated C28/I2 cells. Mechanically, TUG1 sponged miR-320c and miR-320c targeted FUT4. In addition, TUG1 knockdown accelerated cell proliferation and repressed apoptosis and extracellular matrix (ECM) degradation in IL-1β-induced C28/I2 cells, whereas these effects of TUG1 deletion were rescued by either miR-320c inhibitor or FUT4 upregulation. Meanwhile, TUG1 sponged miR-320c to regulate FUT4 expression in IL-1β-induced C28/I2 cells. Collectively, TUG1 modulated cell proliferation, apoptosis and ECM degradation in IL-1β-induced C28/I2 cells via the miR-320c/FUT4 axis, providing a new insight into the OA treatment. |
topic |
osteoarthritis tug1 mir-320c fut4 il-1β |
url |
https://doi.org/10.1515/biol-2021-0037 |
work_keys_str_mv |
AT hanhu longnoncodingrnatug1regulatesdegradationofchondrocyteextracellularmatrixviamir320cmmp13axisinosteoarthritis AT liulijuan longnoncodingrnatug1regulatesdegradationofchondrocyteextracellularmatrixviamir320cmmp13axisinosteoarthritis |
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1716846225358061568 |