Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis
Purpose To investigate the effect of biospray dressing on the extent of eosinophil infiltration in the nasal mucosa and the level of serum IgE in experimental allergic rhinitis with nasal provocation. Method Twenty-four BALB/c mice were randomly divided into the normal control group, allergic rhinit...
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Online Access: | https://doi.org/10.1177/2152656720902142 |
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doaj-d69c884f3a7647729456730c40366d532020-11-25T03:16:19ZengSAGE PublishingAllergy & Rhinology2152-65672020-03-011110.1177/2152656720902142Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic RhinitisShan Huang BScGeping Wu MD, PhDXiaodan Gu BSMHongyan Zhu MDXingkai Ma BSMYifang Yuan BSMYan Lv MDDi Li MDLing Zhang MDPurpose To investigate the effect of biospray dressing on the extent of eosinophil infiltration in the nasal mucosa and the level of serum IgE in experimental allergic rhinitis with nasal provocation. Method Twenty-four BALB/c mice were randomly divided into the normal control group, allergic rhinitis (AR) group, dexamethasone (DEX) treatment group, and biospray dressing (BD) group. The mice in the latter 3 groups were prepared for animal models of AR according to standard protocols. Mice in the BD group were administered a nasal spray before the nasal provocation, and those in the DEX group were administered an intraperitoneal injection of DEX. The nasal mucosa and serum were collected from each group. Nasal mucosa eosinophil infiltration was evaluated using hematoxylin and eosin staining, and enzyme-linked immunosorbent assay (ELISA) was performed to analyze the serum IgE expression. Results Eosinophil infiltration (AR vs BD P = .009) in the nasal mucosa and serum IgE expression (AR vs BD P = .001) were significantly lower in the BD group than in the AR group. There were no significant differences in the extent of eosinophil infiltration in the nasal mucosa or serum IgE expression between the BD and DEX groups. Conclusion Biospray dressings can significantly reduce allergen provocation in the nasal cavity and have a protective effect on the nasal mucosa. They can be used for the prevention and treatment of AR.https://doi.org/10.1177/2152656720902142 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shan Huang BSc Geping Wu MD, PhD Xiaodan Gu BSM Hongyan Zhu MD Xingkai Ma BSM Yifang Yuan BSM Yan Lv MD Di Li MD Ling Zhang MD |
spellingShingle |
Shan Huang BSc Geping Wu MD, PhD Xiaodan Gu BSM Hongyan Zhu MD Xingkai Ma BSM Yifang Yuan BSM Yan Lv MD Di Li MD Ling Zhang MD Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis Allergy & Rhinology |
author_facet |
Shan Huang BSc Geping Wu MD, PhD Xiaodan Gu BSM Hongyan Zhu MD Xingkai Ma BSM Yifang Yuan BSM Yan Lv MD Di Li MD Ling Zhang MD |
author_sort |
Shan Huang BSc |
title |
Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis |
title_short |
Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis |
title_full |
Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis |
title_fullStr |
Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis |
title_full_unstemmed |
Effect of Biospray Dressings on Eosinophil Infiltration in the Nasal Mucosa and Serum IgE Levels After Nasal Provocation in Experimental Allergic Rhinitis |
title_sort |
effect of biospray dressings on eosinophil infiltration in the nasal mucosa and serum ige levels after nasal provocation in experimental allergic rhinitis |
publisher |
SAGE Publishing |
series |
Allergy & Rhinology |
issn |
2152-6567 |
publishDate |
2020-03-01 |
description |
Purpose To investigate the effect of biospray dressing on the extent of eosinophil infiltration in the nasal mucosa and the level of serum IgE in experimental allergic rhinitis with nasal provocation. Method Twenty-four BALB/c mice were randomly divided into the normal control group, allergic rhinitis (AR) group, dexamethasone (DEX) treatment group, and biospray dressing (BD) group. The mice in the latter 3 groups were prepared for animal models of AR according to standard protocols. Mice in the BD group were administered a nasal spray before the nasal provocation, and those in the DEX group were administered an intraperitoneal injection of DEX. The nasal mucosa and serum were collected from each group. Nasal mucosa eosinophil infiltration was evaluated using hematoxylin and eosin staining, and enzyme-linked immunosorbent assay (ELISA) was performed to analyze the serum IgE expression. Results Eosinophil infiltration (AR vs BD P = .009) in the nasal mucosa and serum IgE expression (AR vs BD P = .001) were significantly lower in the BD group than in the AR group. There were no significant differences in the extent of eosinophil infiltration in the nasal mucosa or serum IgE expression between the BD and DEX groups. Conclusion Biospray dressings can significantly reduce allergen provocation in the nasal cavity and have a protective effect on the nasal mucosa. They can be used for the prevention and treatment of AR. |
url |
https://doi.org/10.1177/2152656720902142 |
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