The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines
Chronic alcohol consumption has been identified as a significant risk factor for cancer in humans. The aim of the study was to analyze the influence of low concentrations of ethanol on gastric adenocarcinoma cell viability, apoptosis, and changes in the expression of alcohol dehydrogenase w...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2014-01-01
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Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401317W.pdf |
Summary: | Chronic alcohol consumption has been identified as a significant risk factor
for cancer in humans. The aim of the study was to analyze the influence of
low concentrations of ethanol on gastric adenocarcinoma cell viability,
apoptosis, and changes in the expression of alcohol dehydrogenase with
ethanol treatment. Gastric adenocarcinoma cell lines (MGC803, MGC823 and
SGC7901) were treated with different concentrations of ethanol (0.03125%,
0.0625%, 0.125%, 0.25%, 0.5%, 1%, 2%, and 4%). An MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow
cytometry were used to analyze the effect of ethanol treatment on cell
viability and apoptosis. Western blotting was used to analyze the expression
of alcohol dehydrogenase in gastric carcinoma cells. Ethanol treatment
inhibited cell proliferation in gastric adenocarcinoma cell lines in a
significant dose-dependent manner. Ethanol was also able to induce the
apoptosis of gastric adenocarcinoma cells in a dose-dependent manner. Alcohol
dehydrogenase activity of gastric adenocarcinoma cells increased with the
increase in the concentration of ethanol. Ethanol inhibited cell viability
and growth of gastric adenocarcinoma cell lines. Low concentrations of
ethanol also induced apoptosis and increased the expression of alcohol
dehydrogenase of the gastric adenocarcinoma cell lines. |
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ISSN: | 0354-4664 1821-4339 |