The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines
Chronic alcohol consumption has been identified as a significant risk factor for cancer in humans. The aim of the study was to analyze the influence of low concentrations of ethanol on gastric adenocarcinoma cell viability, apoptosis, and changes in the expression of alcohol dehydrogenase w...
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University of Belgrade, University of Novi Sad
2014-01-01
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doaj-d6aad981a6624eb58368f26655f470d82020-11-24T23:01:01ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392014-01-0166131732110.2298/ABS1401317W0354-46641401317WThe effect of low concentrations of ethanol on gastric adenocarcinoma cell linesWu Lingjiao0Chen Shaohua1Zhang Yu2Pan Hongming3State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China + Department of Oncology, Zhejiang Hospital, Hangzhou, ChinaDepartment of Oncology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, ChinaChronic alcohol consumption has been identified as a significant risk factor for cancer in humans. The aim of the study was to analyze the influence of low concentrations of ethanol on gastric adenocarcinoma cell viability, apoptosis, and changes in the expression of alcohol dehydrogenase with ethanol treatment. Gastric adenocarcinoma cell lines (MGC803, MGC823 and SGC7901) were treated with different concentrations of ethanol (0.03125%, 0.0625%, 0.125%, 0.25%, 0.5%, 1%, 2%, and 4%). An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry were used to analyze the effect of ethanol treatment on cell viability and apoptosis. Western blotting was used to analyze the expression of alcohol dehydrogenase in gastric carcinoma cells. Ethanol treatment inhibited cell proliferation in gastric adenocarcinoma cell lines in a significant dose-dependent manner. Ethanol was also able to induce the apoptosis of gastric adenocarcinoma cells in a dose-dependent manner. Alcohol dehydrogenase activity of gastric adenocarcinoma cells increased with the increase in the concentration of ethanol. Ethanol inhibited cell viability and growth of gastric adenocarcinoma cell lines. Low concentrations of ethanol also induced apoptosis and increased the expression of alcohol dehydrogenase of the gastric adenocarcinoma cell lines.http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401317W.pdfethanolgastric adenocarcinomaapoptosisalcohol dehydrogenase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wu Lingjiao Chen Shaohua Zhang Yu Pan Hongming |
spellingShingle |
Wu Lingjiao Chen Shaohua Zhang Yu Pan Hongming The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines Archives of Biological Sciences ethanol gastric adenocarcinoma apoptosis alcohol dehydrogenase |
author_facet |
Wu Lingjiao Chen Shaohua Zhang Yu Pan Hongming |
author_sort |
Wu Lingjiao |
title |
The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
title_short |
The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
title_full |
The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
title_fullStr |
The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
title_full_unstemmed |
The effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
title_sort |
effect of low concentrations of ethanol on gastric adenocarcinoma cell lines |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 1821-4339 |
publishDate |
2014-01-01 |
description |
Chronic alcohol consumption has been identified as a significant risk factor
for cancer in humans. The aim of the study was to analyze the influence of
low concentrations of ethanol on gastric adenocarcinoma cell viability,
apoptosis, and changes in the expression of alcohol dehydrogenase with
ethanol treatment. Gastric adenocarcinoma cell lines (MGC803, MGC823 and
SGC7901) were treated with different concentrations of ethanol (0.03125%,
0.0625%, 0.125%, 0.25%, 0.5%, 1%, 2%, and 4%). An MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow
cytometry were used to analyze the effect of ethanol treatment on cell
viability and apoptosis. Western blotting was used to analyze the expression
of alcohol dehydrogenase in gastric carcinoma cells. Ethanol treatment
inhibited cell proliferation in gastric adenocarcinoma cell lines in a
significant dose-dependent manner. Ethanol was also able to induce the
apoptosis of gastric adenocarcinoma cells in a dose-dependent manner. Alcohol
dehydrogenase activity of gastric adenocarcinoma cells increased with the
increase in the concentration of ethanol. Ethanol inhibited cell viability
and growth of gastric adenocarcinoma cell lines. Low concentrations of
ethanol also induced apoptosis and increased the expression of alcohol
dehydrogenase of the gastric adenocarcinoma cell lines. |
topic |
ethanol gastric adenocarcinoma apoptosis alcohol dehydrogenase |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2014/0354-46641401317W.pdf |
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