Generation of two human isogenic iPSC lines from fetal dermal fibroblasts
Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenc...
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doaj-d6ba2af2bdb0412d98580b61f68914cc2020-11-24T21:43:50ZengElsevierStem Cell Research1873-50612018-12-0133120124Generation of two human isogenic iPSC lines from fetal dermal fibroblastsRashmi Tandon0Björn Brändl1Natalya Baryshnikova2Alexandro Landshammer3Laura Steenpaß4Oliver Keminer5Ole Pless6Franz-Josef Müller7Zentrum für Integrative Psychiatrie, University Hospital Schleswig-Holstein, Kiel, GermanyZentrum für Integrative Psychiatrie, University Hospital Schleswig-Holstein, Kiel, GermanyZentrum für Integrative Psychiatrie, University Hospital Schleswig-Holstein, Kiel, GermanyMax-Planck-Institute for Molecular Genetics, Berlin, GermanyInstitute of Human Genetics, University Hospital Essen, GermanyFraunhofer IME ScreeningPort, Hamburg, GermanyFraunhofer IME ScreeningPort, Hamburg, GermanyZentrum für Integrative Psychiatrie, University Hospital Schleswig-Holstein, Kiel, Germany; Max-Planck-Institute for Molecular Genetics, Berlin, Germany; Corresponding author at: Zentrum für Integrative Psychiatrie, University Hospital Schleswig-Holstein, Kiel, Germany.Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs.Resource tableUnlabelled TableUnique stem cell lines identifierZIPi013-BZIPi013-EAlternative names of stem cell linesZIP13K2 (ZIPi013-B)ZIP13K5 (ZIPi013-E)InstitutionZentrum für Integrative Psychiatrie gGmbH, Kiel, GermanyContact information of distributorPD Dr. Franz-Josef Müller, franz-josef.mueller@uksh.deType of cell linesiPSCOriginhumanCell SourceFibroblastsClonalityClonalMethod of reprogrammingTransgene free, episomalMultiline rationaleIsogenic clonesGene modificationNOType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateStock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017Cell line repository/bankN/AEthical approvalhttps://www.sciencellonline.com/technical-support/ethical-statement.htmlEthikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu Kiel, approval number A145/11http://www.sciencedirect.com/science/article/pii/S1873506118302472 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rashmi Tandon Björn Brändl Natalya Baryshnikova Alexandro Landshammer Laura Steenpaß Oliver Keminer Ole Pless Franz-Josef Müller |
spellingShingle |
Rashmi Tandon Björn Brändl Natalya Baryshnikova Alexandro Landshammer Laura Steenpaß Oliver Keminer Ole Pless Franz-Josef Müller Generation of two human isogenic iPSC lines from fetal dermal fibroblasts Stem Cell Research |
author_facet |
Rashmi Tandon Björn Brändl Natalya Baryshnikova Alexandro Landshammer Laura Steenpaß Oliver Keminer Ole Pless Franz-Josef Müller |
author_sort |
Rashmi Tandon |
title |
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts |
title_short |
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts |
title_full |
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts |
title_fullStr |
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts |
title_full_unstemmed |
Generation of two human isogenic iPSC lines from fetal dermal fibroblasts |
title_sort |
generation of two human isogenic ipsc lines from fetal dermal fibroblasts |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 |
publishDate |
2018-12-01 |
description |
Two isogenic hiPSC lines, ZIPi013-B and ZIPi013-E, were generated by reprogramming fetal dermal fibroblasts with episomal vectors. Previously, the same fetal fibroblasts were reprogrammed multiple times in a study comparing other reprogramming methods. As a consequence, the genomes have been sequenced multiple times. Both new cell lines offer the opportunity to study basic stem cell biology and model human disease. They can be applied as reference cell lines for creating isogenic clones bearing disease mutations on a well-characterized genomic background, as both cell lines have demonstrated excellent differentiation capacity in multiple labs.Resource tableUnlabelled TableUnique stem cell lines identifierZIPi013-BZIPi013-EAlternative names of stem cell linesZIP13K2 (ZIPi013-B)ZIP13K5 (ZIPi013-E)InstitutionZentrum für Integrative Psychiatrie gGmbH, Kiel, GermanyContact information of distributorPD Dr. Franz-Josef Müller, franz-josef.mueller@uksh.deType of cell linesiPSCOriginhumanCell SourceFibroblastsClonalityClonalMethod of reprogrammingTransgene free, episomalMultiline rationaleIsogenic clonesGene modificationNOType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateStock date ZIPi013-B 8th December 2017, stock date ZIPi013-E 12th December 2017Cell line repository/bankN/AEthical approvalhttps://www.sciencellonline.com/technical-support/ethical-statement.htmlEthikkommission der medizinischen Fakultät der Christian-Albrechts-Universität zu Kiel, approval number A145/11 |
url |
http://www.sciencedirect.com/science/article/pii/S1873506118302472 |
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