Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates
<i>Pseudomonas aeruginosa</i> can alter its lifestyle in response to changes in environmental conditions. The switch to a pathogenic host-associated lifestyle can be triggered by the luminosity settings, resorting to at least one photoreceptor which senses light and regulates cellular pr...
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doaj-d6bdd4b7823c403ababaf6c17d4eb0e72021-09-25T23:45:39ZengMDPI AGBiology2079-77372021-08-011083783710.3390/biology10090837Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical IsolatesCristina S. Mesquita0Artur Ribeiro1Andreia C. Gomes2Pedro M. Santos3CBMA—Centre of Molecular and Environmental Biology, Department of Biology, Campus of Gualtar, University of Minho, 4710-057 Braga, PortugalCEB—Centre of Biological Engineering, Campus of Gualtar, University of Minho, 4710-057 Braga, PortugalCBMA—Centre of Molecular and Environmental Biology, Department of Biology, Campus of Gualtar, University of Minho, 4710-057 Braga, PortugalCBMA—Centre of Molecular and Environmental Biology, Department of Biology, Campus of Gualtar, University of Minho, 4710-057 Braga, Portugal<i>Pseudomonas aeruginosa</i> can alter its lifestyle in response to changes in environmental conditions. The switch to a pathogenic host-associated lifestyle can be triggered by the luminosity settings, resorting to at least one photoreceptor which senses light and regulates cellular processes. This study aimed to address how light exposure affects the dynamic and adaptability of two <i>P. aeruginosa</i> pneumonia-associated isolates, HB13 and HB15. A phenotypic characterization of two opposing growth conditions, constant illumination and intensity of full-spectrum light and total absence of light, was performed. Given the nature of <i>P. aeruginosa</i> pathogenicity, distinct fractions were characterized, and its inherent pathogenic potential screened by comparing induced morphological alterations and cytotoxicity against human pulmonary epithelial cells (A549 cell line). Growth in the dark promoted some virulence-associated traits (e.g., pigment production, LasA proteolytic activity), which, together with higher cytotoxicity of secreted fractions, supported an increased pathogenic potential in conditions that better mimic the lung microenvironment of <i>P. aeruginosa</i>. These preliminary findings evidenced that light exposure settings may influence the <i>P. aeruginosa</i> pathogenic potential, likely owing to differential production of virulence factors. Thus, this study raised awareness towards the importance in controlling light conditions during bacterial pathogenicity evaluation approaches, to more accurately interpret bacterial responses.https://www.mdpi.com/2079-7737/10/9/837cytotoxicityhost–pathogen interactionlight exposurepathogenicityphotobiology<i>Pseudomonas aeruginosa</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristina S. Mesquita Artur Ribeiro Andreia C. Gomes Pedro M. Santos |
spellingShingle |
Cristina S. Mesquita Artur Ribeiro Andreia C. Gomes Pedro M. Santos Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates Biology cytotoxicity host–pathogen interaction light exposure pathogenicity photobiology <i>Pseudomonas aeruginosa</i> |
author_facet |
Cristina S. Mesquita Artur Ribeiro Andreia C. Gomes Pedro M. Santos |
author_sort |
Cristina S. Mesquita |
title |
Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates |
title_short |
Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates |
title_full |
Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates |
title_fullStr |
Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates |
title_full_unstemmed |
Absence of Light Exposure Increases Pathogenicity of <i>Pseudomonas aeruginosa</i> Pneumonia-Associated Clinical Isolates |
title_sort |
absence of light exposure increases pathogenicity of <i>pseudomonas aeruginosa</i> pneumonia-associated clinical isolates |
publisher |
MDPI AG |
series |
Biology |
issn |
2079-7737 |
publishDate |
2021-08-01 |
description |
<i>Pseudomonas aeruginosa</i> can alter its lifestyle in response to changes in environmental conditions. The switch to a pathogenic host-associated lifestyle can be triggered by the luminosity settings, resorting to at least one photoreceptor which senses light and regulates cellular processes. This study aimed to address how light exposure affects the dynamic and adaptability of two <i>P. aeruginosa</i> pneumonia-associated isolates, HB13 and HB15. A phenotypic characterization of two opposing growth conditions, constant illumination and intensity of full-spectrum light and total absence of light, was performed. Given the nature of <i>P. aeruginosa</i> pathogenicity, distinct fractions were characterized, and its inherent pathogenic potential screened by comparing induced morphological alterations and cytotoxicity against human pulmonary epithelial cells (A549 cell line). Growth in the dark promoted some virulence-associated traits (e.g., pigment production, LasA proteolytic activity), which, together with higher cytotoxicity of secreted fractions, supported an increased pathogenic potential in conditions that better mimic the lung microenvironment of <i>P. aeruginosa</i>. These preliminary findings evidenced that light exposure settings may influence the <i>P. aeruginosa</i> pathogenic potential, likely owing to differential production of virulence factors. Thus, this study raised awareness towards the importance in controlling light conditions during bacterial pathogenicity evaluation approaches, to more accurately interpret bacterial responses. |
topic |
cytotoxicity host–pathogen interaction light exposure pathogenicity photobiology <i>Pseudomonas aeruginosa</i> |
url |
https://www.mdpi.com/2079-7737/10/9/837 |
work_keys_str_mv |
AT cristinasmesquita absenceoflightexposureincreasespathogenicityofipseudomonasaeruginosaipneumoniaassociatedclinicalisolates AT arturribeiro absenceoflightexposureincreasespathogenicityofipseudomonasaeruginosaipneumoniaassociatedclinicalisolates AT andreiacgomes absenceoflightexposureincreasespathogenicityofipseudomonasaeruginosaipneumoniaassociatedclinicalisolates AT pedromsantos absenceoflightexposureincreasespathogenicityofipseudomonasaeruginosaipneumoniaassociatedclinicalisolates |
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