O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1.
O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) is the only enzyme catalyzing O-GlcNAcylation. Although it has been shown that OGT plays an essential role in maintaining postnatal heart function, its role in heart development remains unknown. Here we showed that loss of OGT in early fetal ca...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-04-01
|
Series: | PLoS Genetics |
Online Access: | https://doi.org/10.1371/journal.pgen.1008730 |
id |
doaj-d6d9c9f401734777af729cf91bdea453 |
---|---|
record_format |
Article |
spelling |
doaj-d6d9c9f401734777af729cf91bdea4532021-04-21T13:54:09ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042020-04-01164e100873010.1371/journal.pgen.1008730O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1.Yongxin MuHouzhi YuTongbin WuJianlin ZhangSylvia M EvansJu ChenO-linked N-acetylglucosamine (GlcNAc) transferase (OGT) is the only enzyme catalyzing O-GlcNAcylation. Although it has been shown that OGT plays an essential role in maintaining postnatal heart function, its role in heart development remains unknown. Here we showed that loss of OGT in early fetal cardiomyocytes led to multiple heart developmental defects including hypertrabeculation, biventricular dilation, atrial septal defects, ventricular septal defects, and defects in coronary vessel development. In addition, RNA sequencing revealed that Angiopoietin-1, required within cardiomyocytes for both myocardial and coronary vessel development, was dramatically downregulated in cardiomyocyte-specific OGT knockout mouse hearts. In conclusion, our data demonstrated that OGT plays an essential role in regulating heart development through activating expression of cardiomyocyte Angiopoietin-1.https://doi.org/10.1371/journal.pgen.1008730 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yongxin Mu Houzhi Yu Tongbin Wu Jianlin Zhang Sylvia M Evans Ju Chen |
spellingShingle |
Yongxin Mu Houzhi Yu Tongbin Wu Jianlin Zhang Sylvia M Evans Ju Chen O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. PLoS Genetics |
author_facet |
Yongxin Mu Houzhi Yu Tongbin Wu Jianlin Zhang Sylvia M Evans Ju Chen |
author_sort |
Yongxin Mu |
title |
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
title_short |
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
title_full |
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
title_fullStr |
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
title_full_unstemmed |
O-linked β-N-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
title_sort |
o-linked β-n-acetylglucosamine transferase plays an essential role in heart development through regulating angiopoietin-1. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2020-04-01 |
description |
O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) is the only enzyme catalyzing O-GlcNAcylation. Although it has been shown that OGT plays an essential role in maintaining postnatal heart function, its role in heart development remains unknown. Here we showed that loss of OGT in early fetal cardiomyocytes led to multiple heart developmental defects including hypertrabeculation, biventricular dilation, atrial septal defects, ventricular septal defects, and defects in coronary vessel development. In addition, RNA sequencing revealed that Angiopoietin-1, required within cardiomyocytes for both myocardial and coronary vessel development, was dramatically downregulated in cardiomyocyte-specific OGT knockout mouse hearts. In conclusion, our data demonstrated that OGT plays an essential role in regulating heart development through activating expression of cardiomyocyte Angiopoietin-1. |
url |
https://doi.org/10.1371/journal.pgen.1008730 |
work_keys_str_mv |
AT yongxinmu olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 AT houzhiyu olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 AT tongbinwu olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 AT jianlinzhang olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 AT sylviamevans olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 AT juchen olinkedbnacetylglucosaminetransferaseplaysanessentialroleinheartdevelopmentthroughregulatingangiopoietin1 |
_version_ |
1714668586882039808 |