DNA End Resection: Facts and
DNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generat...
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doaj-d6e2e21b39154f70ae93d5e926cabeec2020-11-24T23:00:05ZengElsevierGenomics, Proteomics & Bioinformatics1672-02292016-06-0114312613010.1016/j.gpb.2016.05.002DNA End Resection: Facts andTing Liu0Jun Huang1Department of Cell Biology and Program in Molecular Cell Biology, Zhejiang University School of Medicine, Hangzhou 310058, ChinaLife Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, ChinaDNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generates a long 3′ single-stranded DNA (ssDNA) tail that can invade the homologous DNA strand. The generation of 3′ ssDNA is not only essential for HR repair, but also promotes activation of the ataxia telangiectasia and Rad3-related protein (ATR). Multiple factors, including the MRN/X complex, C-terminal-binding protein interacting protein (CtIP)/Sae2, exonuclease 1 (EXO1), Bloom syndrome protein (BLM)/Sgs1, DNA2 nuclease/helicase, and several chromatin remodelers, cooperate to complete the process of end resection. Here we review the basic machinery involved in DNA end resection in eukaryotic cells.http://www.sciencedirect.com/science/article/pii/S1672022916300717DNA end resectionHomologous recombinationDNA double-strand breaksChromatin remodeling factorsGenome stability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ting Liu Jun Huang |
spellingShingle |
Ting Liu Jun Huang DNA End Resection: Facts and Genomics, Proteomics & Bioinformatics DNA end resection Homologous recombination DNA double-strand breaks Chromatin remodeling factors Genome stability |
author_facet |
Ting Liu Jun Huang |
author_sort |
Ting Liu |
title |
DNA End Resection: Facts and |
title_short |
DNA End Resection: Facts and |
title_full |
DNA End Resection: Facts and |
title_fullStr |
DNA End Resection: Facts and |
title_full_unstemmed |
DNA End Resection: Facts and |
title_sort |
dna end resection: facts and |
publisher |
Elsevier |
series |
Genomics, Proteomics & Bioinformatics |
issn |
1672-0229 |
publishDate |
2016-06-01 |
description |
DNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generates a long 3′ single-stranded DNA (ssDNA) tail that can invade the homologous DNA strand. The generation of 3′ ssDNA is not only essential for HR repair, but also promotes activation of the ataxia telangiectasia and Rad3-related protein (ATR). Multiple factors, including the MRN/X complex, C-terminal-binding protein interacting protein (CtIP)/Sae2, exonuclease 1 (EXO1), Bloom syndrome protein (BLM)/Sgs1, DNA2 nuclease/helicase, and several chromatin remodelers, cooperate to complete the process of end resection. Here we review the basic machinery involved in DNA end resection in eukaryotic cells. |
topic |
DNA end resection Homologous recombination DNA double-strand breaks Chromatin remodeling factors Genome stability |
url |
http://www.sciencedirect.com/science/article/pii/S1672022916300717 |
work_keys_str_mv |
AT tingliu dnaendresectionfactsand AT junhuang dnaendresectionfactsand |
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